Pseudomonas aeruginosa PAO1, PA5337 (rpoZ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5337
Name
rpoZ
Replicon chromosome
Genomic location 6005899 - 6006165 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_254024.1
GI 15600530
Affymetrix PA5337_rpoZ_at
DNASU PaCD00008307
Entrez 878048
GenBank AAG08722.1
INSDC AAG08722.1
NCBI Locus Tag PA5337
protein_id(GenBank) gb|AAG08722.1|AE004946_6|gnl|PseudoCAP|PA5337
TIGR NTL03PA05338
UniParc UPI0000134801
UniProtKB Acc Q9HTM1
UniProtKB ID RPOZ_PSEAE
UniRef100 UniRef100_A6VEE4
UniRef50 UniRef50_Q5E8P6
UniRef90 UniRef90_A6VEE4

Product

Feature Type CDS
Coding Frame 1
Product Name
RNA polymerase omega subunit
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.03
Kyte-Doolittle Hydrophobicity Value -0.309
Molecular Weight (kDa) 9.8
Isoelectric Point (pI) 3.95

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7XYA TRANSCRIPTION 06/01/22 The cryo-EM structure of an AlpA-loading complex Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.3 ELECTRON MICROSCOPY 100.0
7XYB TRANSCRIPTION 06/01/22 The cryo-EM structure of an AlpA-loaded complex Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.7 ELECTRON MICROSCOPY 100.0
7XL4 TRANSCRIPTION 04/21/22 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (closed lobe) Pseudomonas aeruginosa PAO1 3.86 ELECTRON MICROSCOPY 100.0
7XL3 TRANSCRIPTION 04/21/22 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (open lobe) Pseudomonas aeruginosa PAO1 3.13 ELECTRON MICROSCOPY 100.0
7VF9 TRANSCRIPTION 09/10/21 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes Pseudomonas aeruginosa PAO1 4.04 ELECTRON MICROSCOPY 100.0
7F0R TRANSCRIPTION/DNA 06/06/21 Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 5.8 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 245 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004906 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5337
Search term: rpoZ

Human Homologs

References

The spoU gene of Escherichia coli, the fourth gene of the spoT operon, is essential for tRNA (Gm18) 2'-O-methyltransferase activity.
Persson BC, Jäger G, Gustafsson C
Nucleic Acids Res 1997 Oct 15;25(20):4093-7
PubMed ID: 9321663