Pseudomonas aeruginosa PAO1, PA5338 (spoT)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 6006231 - 6008336 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_254025.1
GI 15600531
Affymetrix PA5338_spoT_at
Entrez 877912
GenBank AAG08723.1
INSDC AAG08723.1
NCBI Locus Tag PA5338
protein_id(GenBank) gb|AAG08723.1|AE004946_7|gnl|PseudoCAP|PA5338
UniParc UPI00000C5FAE
UniProtKB Acc Q9HTM0
UniRef100 UniRef100_Q9HTM0
UniRef50 UniRef50_D7HTS2
UniRef90 UniRef90_D7HTS2


Feature Type CDS
Coding Frame 1
Product Name
guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
Product Name Confidence: Class 2
Synonyms (p)ppGpp synthetase II
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 17.81
Kyte-Doolittle Hydrophobicity Value -0.275
Molecular Weight (kDa) 78.9
Isoelectric Point (pI) 9.35

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category Stringent Response
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Drosophila melanogaster
Notes spoT relA double mutant was significantly less virulent than both wild-type strain PAO1 and the relA mutant in D. melanogaster feeding assay.
Infection model Drosophila melanogaster feeding assay
Reference 21788391
Source 2
Database PseudoCAP
Category Stringent Response
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Rattus norvegicus
Notes spoT relA double mutant was avirulent in rat lung agar bead model of infection in which the relA mutant is fully virulent.
Infection model rat lung agar bead model of infection
Reference 21788391

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 575 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004907 (536 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA5338
Search term: spoT

Human Homologs


Residual guanosine 3',5'-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoT null mutations.
Xiao H, Kalman M, Ikehara K, Zemel S, Glaser G, Cashel M
J Biol Chem 1991 Mar 25;266(9):5980-90
PubMed ID: 2005134