Pseudomonas aeruginosa PAO1, PA5353 (glcF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5353
Name
glcF
Replicon chromosome
Genomic location 6019888 - 6021114 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_254040.1
GI 15600546
Affymetrix PA5353_glcF_at
Entrez 877710
GenBank AAG08738.1
INSDC AAG08738.1
NCBI Locus Tag PA5353
protein_id(GenBank) gb|AAG08738.1|AE004947_8|gnl|PseudoCAP|PA5353
TIGR NTL03PA05354
UniParc UPI00000C5FBC
UniProtKB Acc Q9HTK5
UniProtKB ID Q9HTK5_PSEAE
UniRef100 UniRef100_Q9HTK5
UniRef50 UniRef50_Q4KEF9
UniRef90 UniRef90_Q9HTK5

Product

Feature Type CDS
Coding Frame 1
Product Name
glycolate oxidase subunit GlcF
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 8.08
Kyte-Doolittle Hydrophobicity Value -0.299
Molecular Weight (kDa) 44.7
Isoelectric Point (pI) 8.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 433 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004919 (475 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5353
Search term: glcF

Human Homologs

References

Cross-induction of glc and ace operons of Escherichia coli attributable to pathway intersection. Characterization of the glc promoter.
Pellicer MT, Fernandez C, Badía J, Aguilar J, Lin EC, Baldom L
J Biol Chem 1999 Jan 15;274(3):1745-52
PubMed ID: 9880556