Pseudomonas aeruginosa PAO1, PA5373 (betB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5373
Name
betB
Replicon chromosome
Genomic location 6049185 - 6050657 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_254060.1
GI 15600566
Affymetrix PA5373_betB_at
Entrez 881619
GenBank AAG08758.1
INSDC AAG08758.1
NCBI Locus Tag PA5373
protein_id(GenBank) gb|AAG08758.1|AE004950_1|gnl|PseudoCAP|PA5373
TIGR NTL03PA05374
UniParc UPI00000C5FCA
UniProtKB Acc Q9HTJ1
UniProtKB ID BETB_PSEAE
UniRef100 UniRef100_B7V5R4
UniRef50 UniRef50_Q8FKI8
UniRef90 UniRef90_B7V5R4

Product

Feature Type CDS
Coding Frame 1
Product Name
betaine aldehyde dehydrogenase
Product Name Confidence: Class 2
Synonyms betB protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.65
Kyte-Doolittle Hydrophobicity Value -0.146
Molecular Weight (kDa) 53.3
Isoelectric Point (pI) 5.01

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2WOX OXIDOREDUCTASE 07/30/09 Betaine aldehyde dehydrogenase from Pseudomonas aeruginosa with NAD(P) H-catalytic thiol adduct. PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 100.0
2WME OXIDOREDUCTASE 06/30/09 Crystallographic structure of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 100.0
6BJP OXIDOREDUCTASE 11/06/17 Apo form of the E124S mutant of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.102 X-RAY DIFFRACTION 99.8
4CAZ OXIDOREDUCTASE 10/09/13 CRYSTAL STRUCTURE OF BETAINE ALDEHYDE DEHYDROGENASE FROM Pseudomonas aeruginosa IN COMPLEX WITH NADH PSEUDOMONAS AERUGINOSA 2.55 X-RAY DIFFRACTION 100.0
6BPG OXIDOREDUCTASE 11/23/17 Crystal structure of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa with bound rubidium ions Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.086 X-RAY DIFFRACTION 100.0
4CBB OXIDOREDUCTASE 10/11/13 APO FORM OF BETAINE ALDEHYDE DEHYDROGENASE FROM Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 607 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004938 (527 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5373
Search term: betB

Human Homologs

Ensembl 110, assembly GRCh38.p14
aldehyde dehydrogenase 9 family member A1 [Source:HGNC Symbol;Acc:HGNC:412]
E-value: 5.7e-141
Percent Identity: 52.9

References

Characterization of an Escherichia coli gene encoding betaine aldehyde dehydrogenase (BADH): structural similarity to mammalian ALDHs and a plant BADH.
Boyd LA, Adam L, Pelcher LE, McHughen A, Hirji R, Selvaraj G
Gene 1991 Jul 15;103(1):45-52
PubMed ID: 1879697