Pseudomonas aeruginosa PAO1, PA5560 (atpB)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: papD
Synonym: uncB
Replicon chromosome
Genomic location 6253542 - 6254411 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_254247.1
GI 15600753
Affymetrix PA5560_atpB_at
DNASU PaCD00007318
Entrez 880089
GenBank AAG08945.1
INSDC AAG08945.1
NCBI Locus Tag PA5560
protein_id(GenBank) gb|AAG08945.1|AE004967_16|gnl|PseudoCAP|PA5560
UniParc UPI00000D42A1
UniProtKB Acc Q9HT14
UniRef100 UniRef100_A6VF38
UniRef50 UniRef50_Q329S7
UniRef90 UniRef90_A6VF38


Feature Type CDS
Coding Frame 1
Product Name
ATP synthase A chain
Product Name Confidence: Class 2
Evidence for Translation
Detected using LC-MS/MS (31.92 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.61
Kyte-Doolittle Hydrophobicity Value 0.828
Molecular Weight (kDa) 31.9
Isoelectric Point (pI) 6.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 497 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005091 (535 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA5560
Search term: atpB

Human Homologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The nucleotide sequence of the atp genes coding for the F0 subunits a, b, c and the F1 subunit delta of the membrane bound ATP synthase of Escherichia coli.
Nielsen J, Hansen FG, Hoppe J, Friedl P, von Meyenburg K
Mol Gen Genet 1981;184(1):33-9
PubMed ID: 6278247
A topological analysis of subunit alpha from Escherichia coli F1F0-ATP synthase predicts eight transmembrane segments.
Lewis MJ, Chang JA, Simoni RD
J Biol Chem 1990 Jun 25;265(18):10541-50
PubMed ID: 2162353
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344