Pseudomonas aeruginosa PAO1, PA0002 (dnaN)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 2056 - 3159 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_064722.1
GI 15595200
Affymetrix PA0002_dnaN_at
DNASU PaCD00006004
Entrez 879244
GenBank AAG03392.1
INSDC AAG03392.1
NCBI Locus Tag PA0002
protein_id(GenBank) gb|AAG03392.1|AE004440_2|gnl|PseudoCAP|PA0002
UniParc UPI0000129706
UniProtKB Acc Q9I7C4
UniRef100 UniRef100_Q9I7C4
UniRef50 UniRef50_Q9I7C4
UniRef90 UniRef90_Q9I7C4


Feature Type CDS
Coding Frame 1
Product Name
DNA polymerase III, beta chain
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.60
Kyte-Doolittle Hydrophobicity Value -0.147
Molecular Weight (kDa) 40.7
Isoelectric Point (pI) 4.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6PTH TRANSFERASE/ANTIBIOTIC 07/15/19 Crystal structure of a DnaN sliding clamp (DNA polymerase III subunit beta) from Pseudomonas aeruginosa bound to griselimycin Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 3.05 X-RAY DIFFRACTION 100.0
4TSZ TRANSFERASE 06/19/14 Crystal structure of DNA polymerase sliding clamp from Pseudomonas aeruginosa with ligand Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 2 X-RAY DIFFRACTION 100.0
6AMS TRANSFERASE 08/11/17 Crystal structure of the DNA polymerase III subunit beta from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.39 X-RAY DIFFRACTION 100.0
4TR8 DNA BINDING PROTEIN 06/15/14 Crystal structure of DNA polymerase sliding clamp from Pseudomonas aeruginosa Pseudomonas aeruginosa HB15 1.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 588 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000002 (538 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0002
Search term: dnaN

Human Homologs


The dnaN gene codes for the beta subunit of DNA polymerase III holoenzyme of escherichia coli.
Burgers PM, Kornberg A, Sakakibara Y
Proc Natl Acad Sci U S A 1981 Sep;78(9):5391-5
PubMed ID: 6458041
Structural analysis of the dnaA and dnaN genes of Escherichia coli.
Ohmori H, Kimura M, Nagata T, Sakakibara Y
Gene 1984 May;28(2):159-70
PubMed ID: 6234204
Three-dimensional structure of the beta subunit of E. coli DNA polymerase III holoenzyme: a sliding DNA clamp.
Kong XP, Onrust R, O'Donnell M, Kuriyan J
Cell 1992 May 1;69(3):425-37
PubMed ID: 1349852