Pseudomonas aeruginosa PAO1, PA0337 (ptsP)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 378598 - 380877 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs
CommentptsP has an important role in the negative regulation of pyocyanin production via the expression of qscR.


RefSeq NP_249028.1
GI 15595534
Affymetrix PA0337_ptsP_at
Entrez 882232
GenBank AAG03726.1
INSDC AAG03726.1
NCBI Locus Tag PA0337
protein_id(GenBank) gb|AAG03726.1|AE004471_9|gnl|PseudoCAP|PA0337
UniParc UPI00000D4254
UniProtKB Acc G3XD36
UniRef100 UniRef100_G3XD36
UniRef50 UniRef50_Q4K476
UniRef90 UniRef90_A0A024HPQ7


Feature Type CDS
Coding Frame 1
Product Name
phosphoenolpyruvate-protein phosphotransferase PtsP
Product Name Confidence: Class 1
Synonyms phosphotransferase system enzyme I
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -14.70
Kyte-Doolittle Hydrophobicity Value -0.047
Molecular Weight (kDa) 83.6
Isoelectric Point (pI) 5.56

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 619 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000329 (534 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0337
Search term: ptsP

Human Homologs


Influence of ptsP gene on pyocyanin production in Pseudomonas aeruginosa.
Xu H, Lin W, Xia H, Xu S, Li Y, Yao H, Bai F, Zhang X, Bai Y, Saris P, Qiao M
FEMS Microbiol Lett 2005 Dec 1;253(1):103-9
PubMed ID: 16239083
Novel phosphotransferase-encoding genes revealed by analysis of the Escherichia coli genome: a chimeric gene encoding an Enzyme I homologue that possesses a putative sensory transduction domain.
Reizer J, Reizer A, Merrick MJ, Plunkett G 3rd, Rose DJ, Saier MH Jr
Gene 1996 Nov 28;181(1-2):103-8
PubMed ID: 8973315
Mutational inactivation of a gene homologous to Escherichia coli ptsP affects poly-beta-hydroxybutyrate accumulation and nitrogen fixation in Azotobacter vinelandii.
Segura D, Espín G
J Bacteriol 1998 Sep;180(18):4790-8
PubMed ID: 9733679