Pseudomonas aeruginosa PAO1, PA3013 (faoB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3013
Name
faoB
Synonym: fadA
Synonym: fadA5
Replicon chromosome
Genomic location 3373254 - 3374429 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentPsrA binds to the promoter region of faoAB. PsrA also binds to and responds to long chain fatty acids to derepress the faoAB operon.

Cross-References

RefSeq NP_251703.1
GI 15598209
Affymetrix PA3013_foaB_at
Entrez 880523
GenBank AAG06401.1
INSDC AAG06401.1
NCBI Locus Tag PA3013
protein_id(GenBank) gb|AAG06401.1|AE004726_7|gnl|PseudoCAP|PA3013
TIGR NTL03PA03013
UniParc UPI00000C58C8
UniProtKB Acc Q9HZJ3
UniProtKB ID FADA_PSEAE
UniRef100 UniRef100_Q9HZJ3
UniRef50 UniRef50_P21151
UniRef90 UniRef90_P28790

Product

Feature Type CDS
Coding Frame 1
Product Name
fatty-acid oxidation complex beta-subunit
Product Name Confidence: Class 2
Synonyms 3-ketoacyl-coA thiolase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.26
Kyte-Doolittle Hydrophobicity Value -0.049
Molecular Weight (kDa) 41.6
Isoelectric Point (pI) 6.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1WDM LYASE,OXIDOREDUCTASE/TRANSFERASE 05/17/04 fatty acid beta-oxidation multienzyme complex from Pseudomonas fragi, form I (native3) Pseudomonas fragi 3.8 X-RAY DIFFRACTION 90.5
2D3T LYASE, OXIDOREDUCTASE/TRANSFERASE 10/01/05 Fatty Acid beta-oxidation multienzyme complex from Pseudomonas Fragi, Form V Pseudomonas fragi 3.4 X-RAY DIFFRACTION 90.5
1WDK LYASE,OXIDOREDUCTASE/TRANSFERASE 05/17/04 fatty acid beta-oxidation multienzyme complex from Pseudomonas fragi, form I (native2) Pseudomonas fragi 2.5 X-RAY DIFFRACTION 90.5
1WDL LYASE,OXIDOREDUCTASE/TRANSFERASE 05/17/04 fatty acid beta-oxidation multienzyme complex from Pseudomonas fragi, form II (native4) Pseudomonas fragi 3.5 X-RAY DIFFRACTION 90.5

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 478 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002569 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3013
Search term: faoB

Human Homologs

Ensembl 110, assembly GRCh38.p14
acetyl-CoA acyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:82]
E-value: 3.2e-62
Percent Identity: 40.4

References

Primary structures of the genes, faoA and faoB, from Pseudomonas fragi B-0771 which encode the two subunits of the HDT multienzyme complex involved in fatty acid beta-oxidation.
Sato S, Hayashi M, Imamura S, Ozeki Y, Kawaguchi A
J Biochem 1992 Jan;111(1):8-15
PubMed ID: 1607366