Pseudomonas aeruginosa PAO1, PA3636 (kdsA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 4070012 - 4070857 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_252326.1
GI 15598832
Affymetrix PA3636_kdsA_at
DNASU PaCD00007596
Entrez 880428
GenBank AAG07024.1
INSDC AAG07024.1
NCBI Locus Tag PA3636
protein_id(GenBank) gb|AAG07024.1|AE004783_9|gnl|PseudoCAP|PA3636
UniParc UPI000012DD5C
UniProtKB Acc Q9ZFK4
UniRef100 UniRef100_Q02RA8
UniRef50 UniRef50_Q88LX0
UniRef90 UniRef90_Q02RA8


Feature Type CDS
Coding Frame 1
Product Name
2-dehydro-3-deoxyphosphooctonate aldolase
Product Name Confidence: Class 1
Synonyms 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
Kdo-8-P synthase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -1.74
Kyte-Doolittle Hydrophobicity Value -0.033
Molecular Weight (kDa) 31.1
Isoelectric Point (pI) 6.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4LU0 TRANSFERASE 07/24/13 Crystal structure of 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa. Pseudomonas aeruginosa 2.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 504 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002038 (537 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA3636
Search term: kdsA

Human Homologs


The kdsA gene coding for 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase is part of an operon in Escherichia coli.
Woisetschläger M, Högenauer G
Mol Gen Genet 1987 May;207(2-3):369-73
PubMed ID: 3039295
Overproduction and one-step purification of Escherichia coli 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase and oxygen transfer studies during catalysis using isotopic-shifted heteronuclear NMR.
Dotson GD, Dua RK, Clemens JC, Wooten EW, Woodard RW
J Biol Chem 1995 Jun 9;270(23):13698-705
PubMed ID: 7775423
Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12.
Strohmaier H, Remler P, Renner W, Högenauer G
J Bacteriol 1995 Aug;177(15):4488-500
PubMed ID: 7543480
Genetic and biochemical characterization of an operon involved in the biosynthesis of 3-deoxy-D-manno-octulosonic acid in Pseudomonas aeruginosa.
Walsh AG, Burrows LL, Lam JS
FEMS Microbiol Lett 1999 Apr 1;173(1):27-33
PubMed ID: 10220877
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994