Pseudomonas aeruginosa PAO1, PA5192 (pckA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 5844468 - 5846009 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_253879.1
GI 15600385
Affymetrix PA5192_pckA_at
Entrez 879312
GenBank AAG08577.1
INSDC AAG08577.1
NCBI Locus Tag PA5192
protein_id(GenBank) gb|AAG08577.1|AE004932_4|gnl|PseudoCAP|PA5192
UniParc UPI000013202F
UniProtKB Acc Q9HTZ7
UniRef100 UniRef100_Q9HTZ7
UniRef50 UniRef50_Q9HTZ7
UniRef90 UniRef90_Q9HTZ7


Feature Type CDS
Coding Frame 1
Product Name
phosphoenolpyruvate carboxykinase
Product Name Confidence: Class 2
Synonyms PEPCK
PEP carboxykinase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected by iTRAQ (PMID:21863142).
Charge (pH 7) -12.06
Kyte-Doolittle Hydrophobicity Value -0.152
Molecular Weight (kDa) 55.7
Isoelectric Point (pI) 5.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 275 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004776 (533 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA5192
Search term: pckA

Human Homologs


Sequence of the pckA gene of Escherichia coli K-12: relevance to genetic and allosteric regulation and homology of E. coli phosphoenolpyruvate carboxykinase with the enzymes from Trypanosoma brucei and Saccharomyces cerevisiae.
Medina V, Pontarollo R, Glaeske D, Tabel H, Goldie H
J Bacteriol 1990 Dec;172(12):7151-6
PubMed ID: 1701430
Molecular and expression analysis of the Rhizobium meliloti phosphoenolpyruvate carboxykinase (pckA) gene.
Osterås M, Driscoll BT, Finan TM
J Bacteriol 1995 Mar;177(6):1452-60
PubMed ID: 7883700
Snapshot of an enzyme reaction intermediate in the structure of the ATP-Mg2+-oxalate ternary complex of Escherichia coli PEP carboxykinase.
Tari LW, Matte A, Pugazhenthi U, Goldie H, Delbaere LT
Nat Struct Biol 1996 Apr;3(4):355-63
PubMed ID: 8599762
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142