Pseudomonas aeruginosa PAO1, PA5493 (polA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 6183784 - 6186525 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_254180.1
GI 15600686
Affymetrix PA5493_polA_at
Entrez 877794
GenBank AAG08878.1
INSDC AAG08878.1
NCBI Locus Tag PA5493
protein_id(GenBank) gb|AAG08878.1|AE004962_2|gnl|PseudoCAP|PA5493
UniParc UPI00000C6035
UniProtKB Acc Q9HT80
UniRef100 UniRef100_Q9HT80
UniRef50 UniRef50_Q9HT80
UniRef90 UniRef90_Q9HT80


Feature Type CDS
Coding Frame 1
Product Name
DNA polymerase I
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -32.14
Kyte-Doolittle Hydrophobicity Value -0.135
Molecular Weight (kDa) 99.8
Isoelectric Point (pI) 4.75

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 592 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005032 (536 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA5493
Search term: polA
Search term: DNA polymerase I

Human Homologs

Ensembl 110, assembly GRCh38.p14
DNA polymerase nu [Source:HGNC Symbol;Acc:HGNC:18870]
E-value: 7.2e-57
Percent Identity: 31.3
Ensembl 110, assembly GRCh38.p14
DNA polymerase nu [Source:HGNC Symbol;Acc:HGNC:18870]
E-value: 7.2e-57
Percent Identity: 31.3


Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I.
Joyce CM, Kelley WS, Grindley ND
J Biol Chem 1982 Feb 25;257(4):1958-64
PubMed ID: 6276402
Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP.
Ollis DL, Brick P, Hamlin R, Xuong NG, Steitz TA
Nature 1985 Feb 28-Mar 6;313(6005):762-6
PubMed ID: 3883192
Structural principles for the inhibition of the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I by phosphorothioates.
Brautigam CA, Steitz TA
J Mol Biol 1998 Mar 27;277(2):363-77
PubMed ID: 9514742
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994