Pseudomonas aeruginosa PAO1, PA0035 (trpA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0035
Name
trpA
Replicon chromosome
Genomic location 37087 - 37893 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248725.1
GI 15595233
Affymetrix PA0035_trpA_at
DNASU PaCD00006017
Entrez 879479
GenBank AAG03425.1
INSDC AAG03425.1
NCBI Locus Tag PA0035
protein_id(GenBank) gb|AAG03425.1|AE004443_3|gnl|PseudoCAP|PA0035
TIGR NTL03PA00036
UniParc UPI00001374E5
UniProtKB Acc P07344
UniProtKB ID TRPA_PSEAE
UniRef100 UniRef100_P07344
UniRef50 UniRef50_P07344
UniRef90 UniRef90_P07344

Product

Feature Type CDS
Coding Frame 1
Product Name
tryptophan synthase alpha chain
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.37
Kyte-Doolittle Hydrophobicity Value 0.061
Molecular Weight (kDa) 28.5
Isoelectric Point (pI) 5.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 581 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000035 (562 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0035
Search term: trpA

References

Nucleotide sequence of the genes for tryptophan synthase in Pseudomonas aeruginosa.
Hadero A, Crawford IP
Mol. Biol. Evol. 1986 May;3(3):191-204
PubMed ID: 3127651
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994