Pseudomonas aeruginosa PAO1, PA0740 (sdsA1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0740
Name
sdsA1
Synonym: yjcS
Synonym: sdsA1
Replicon chromosome
Genomic location 806805 - 808781 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_249431.1
GI 15595937
Affymetrix PA0740_at
Entrez 882069
GenBank AAG04129.1
INSDC AAG04129.1
NCBI Locus Tag PA0740
protein_id(GenBank) gb|AAG04129.1|AE004509_4|gnl|PseudoCAP|PA0740
TIGR NTL03PA00741
UniParc UPI00000C512F
UniProtKB Acc Q9I5I9
UniProtKB ID Q9I5I9_PSEAE
UniRef100 UniRef100_Q9I5I9
UniRef50 UniRef50_Q9I5I9
UniRef90 UniRef90_Q9I5I9

Product

Feature Type CDS
Coding Frame 1
Product Name
SDS hydrolase SdsA1
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -8.84
Kyte-Doolittle Hydrophobicity Value -0.252
Molecular Weight (kDa) 72.6
Isoelectric Point (pI) 5.82

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Extracellular Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5AIJ HYDROLASE 02/13/15 P. aeruginosa SdsA hexagonal polymorph PSEUDOMONAS AERUGINOSA 1.95 X-RAY DIFFRACTION 100.0
2CG2 HYDROLASE 02/27/06 Crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, in complex with sulfate PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 100.0
2CFU HYDROLASE 02/23/06 Crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, in complex with 1-decane-sulfonic-acid. PSEUDOMONAS AERUGINOSA 1.9 X-RAY DIFFRACTION 100.0
5AJL HYDROLASE 02/25/15 Sdsa sulfatase tetragonal PSEUDOMONAS AERUGINOSA 3.45 X-RAY DIFFRACTION 100.0
2CFZ HYDROLASE 02/26/06 Crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, in complex with 1-dodecanol PSEUDOMONAS AERUGINOSA 2.05 X-RAY DIFFRACTION 100.0
5A23 HYDROLASE 05/11/15 SdsA sulfatase triclinic form PSEUDOMONAS AERUGINOSA 2.41 X-RAY DIFFRACTION 100.0
2CG3 HYDROLASE 02/27/06 Crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa. PSEUDOMONAS AERUGINOSA 2.6 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
1-DODECANOL DB06894 DrugBank 5.0.6 Q9I5I9 SDS hydrolase SdsA1 100.0 658 0.0e+00
1-DECANE-SULFONIC-ACID DB06893 DrugBank 5.0.6 Q9I5I9 SDS hydrolase SdsA1 100.0 658 0.0e+00

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 106 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000715 (367 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0740
Search term: sdsA1

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 11402
Genomic Position 807965
Position in ORF 1161(1977)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 42553
Genomic Position 807866
Position in ORF 1062(1977)
Reference 14617778

Transposon Insertions in Orthologs

References

The crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, defines a third class of sulfatases.
Hagelueken G, Adams TM, Wiehlmann L, Widow U, Kolmar H, Tümmler B, Heinz DW, Schubert WD
Proc. Natl. Acad. Sci. U.S.A. 2006 May;103(20):7631-6
PubMed ID: 16684886