Pseudomonas aeruginosa PAO1, PA4762 (grpE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4762
Name
grpE
Replicon chromosome
Genomic location 5349201 - 5349761 (- strand)

Cross-References

RefSeq NP_253450.1
GI 15599956
Affymetrix PA4762_grpE_at
DNASU PaCD00008065
Entrez 881766
GenBank AAG08148.1
INSDC AAG08148.1
NCBI Locus Tag PA4762
protein_id(GenBank) gb|AAG08148.1|AE004890_1|gnl|PseudoCAP|PA4762
TIGR NTL03PA04763
UniParc UPI00000C5DF4
UniProtKB Acc Q9HV42
UniProtKB ID GRPE_PSEAE
UniRef100 UniRef100_Q02FR0
UniRef50 UniRef50_Q02FR0
UniRef90 UniRef90_Q02FR0

Product

Feature Type CDS
Coding Frame 1
Product Name
heat shock protein GrpE
Product Name Confidence: Class 2
Synonyms Hsp24
heat shock protein B25.3
Evidence for Translation
Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -15.51
Kyte-Doolittle Hydrophobicity Value -0.604
Molecular Weight (kDa) 20.7
Isoelectric Point (pI) 4.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 616 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004406 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4762
Search term: grpE

Human Homologs

References

Sequence analysis and transcriptional regulation of the Escherichia coli grpE gene, encoding a heat shock protein.
Lipinska B, King J, Ang D, Georgopoulos C
Nucleic Acids Res 1988 Aug 11;16(15):7545-62
PubMed ID: 3045760
Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK.
Liberek K, Marszalek J, Ang D, Georgopoulos C, Zylicz M
Proc Natl Acad Sci U S A 1991 Apr 1;88(7):2874-8
PubMed ID: 1826368
Structure-function analysis of the Escherichia coli GrpE heat shock protein.
Wu B, Wawrzynow A, Zylicz M, Georgopoulos C
EMBO J 1996 Sep 16;15(18):4806-16
PubMed ID: 8890154
Crystal structure of the nucleotide exchange factor GrpE bound to the ATPase domain of the molecular chaperone DnaK.
Harrison CJ, Hayer-Hartl M, Di Liberto M, Hartl F, Kuriyan J
Science 1997 Apr 18;276(5311):431-5
PubMed ID: 9103205
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142