Pseudomonas aeruginosa PAO1, PA0316 (serA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0316
Name
serA
Replicon chromosome
Genomic location 355248 - 356477 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249007.1
GI 15595513
Affymetrix PA0316_serA_at
Entrez 878305
GenBank AAG03705.1
INSDC AAG03705.1
NCBI Locus Tag PA0316
protein_id(GenBank) gb|AAG03705.1|AE004469_10|gnl|PseudoCAP|PA0316
TIGR NTL03PA00317
UniParc UPI00000C4FFF
UniProtKB Acc Q9I6H5
UniProtKB ID Q9I6H5_PSEAE
UniRef100 UniRef100_W0YLF1
UniRef50 UniRef50_P0A9T2
UniRef90 UniRef90_Q1I3B3

Product

Feature Type CDS
Coding Frame 1
Product Name
PHGDH
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -3.22
Kyte-Doolittle Hydrophobicity Value 0.035
Molecular Weight (kDa) 44.2
Isoelectric Point (pI) 6.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 639 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000313 (561 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0316
Search term: serA
Search term: PHGDH

References

The nucleotide sequence of the serA gene of Escherichia coli and the amino acid sequence of the encoded protein, D-3-phosphoglycerate dehydrogenase.
Tobey KL, Grant GA
J. Biol. Chem. 1986 Sep;261(26):12179-83
PubMed ID: 3017965
The allosteric ligand site in the Vmax-type cooperative enzyme phosphoglycerate dehydrogenase.
Schuller DJ, Grant GA, Banaszak LJ
Nat. Struct. Biol. 1995 Jan;2(1):69-76
PubMed ID: 7719856
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
PubMed ID: 28827360
PubMed ID: 28046014