Pseudomonas aeruginosa PAO1, PA0316 (serA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0316
Name
serA
Replicon chromosome
Genomic location 355248 - 356477 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249007.1
GI 15595513
Affymetrix PA0316_serA_at
Entrez 878305
GenBank AAG03705.1
INSDC AAG03705.1
NCBI Locus Tag PA0316
protein_id(GenBank) gb|AAG03705.1|AE004469_10|gnl|PseudoCAP|PA0316
TIGR NTL03PA00317
UniParc UPI00000C4FFF
UniProtKB Acc Q9I6H5
UniProtKB ID Q9I6H5_PSEAE
UniRef100 UniRef100_W0YLF1
UniRef50 UniRef50_P0A9T2
UniRef90 UniRef90_Q1I3B3

Product

Feature Type CDS
Coding Frame 1
Product Name
PHGDH
Product Name Confidence: Class 1
Synonyms D-3-phosphoglycerate dehydrogenase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -3.22
Kyte-Doolittle Hydrophobicity Value 0.035
Molecular Weight (kDa) 44.2
Isoelectric Point (pI) 6.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 639 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000313 (561 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0316
Search term: serA
Search term: PHGDH

References

The nucleotide sequence of the serA gene of Escherichia coli and the amino acid sequence of the encoded protein, D-3-phosphoglycerate dehydrogenase.
Tobey KL, Grant GA
J. Biol. Chem. 1986 Sep;261(26):12179-83
PubMed ID: 3017965
The allosteric ligand site in the Vmax-type cooperative enzyme phosphoglycerate dehydrogenase.
Schuller DJ, Grant GA, Banaszak LJ
Nat. Struct. Biol. 1995 Jan;2(1):69-76
PubMed ID: 7719856
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Coupling between d-3-phosphoglycerate dehydrogenase and d-2-hydroxyglutarate dehydrogenase drives bacterial l-serine synthesis.
Zhang W, Zhang M, Gao C, Zhang Y, Ge Y, Guo S, Guo X, Zhou Z, Liu Q, Zhang Y, Ma C, Tao F, Xu P.
Proc Natl Acad Sci U S A. 2017 Sep 5;114(36):E7574-E7582.
PubMed ID: 28827360
Pseudomonas aeruginosa serA Gene Is Required for Bacterial Translocation through Caco-2 Cell Monolayers.
Yasuda M, Nagata S, Yamane S, Kunikata C, Kida Y, Kuwano K, Suezawa C, Okuda J.
PLoS One. 2017 Jan 3;12(1):e0169367.
PubMed ID: 28046014