Pseudomonas aeruginosa PAO1, PA0428

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0428
Name
Synonym: rhlE
Replicon chromosome
Genomic location 477886 - 479805 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249119.1
GI 15595625
Affymetrix PA0428_at
Entrez 877822
GenBank AAG03817.1
INSDC AAG03817.1
NCBI Locus Tag PA0428
protein_id(GenBank) gb|AAG03817.1|AE004480_1|gnl|PseudoCAP|PA0428
TIGR NTL03PA00429
UniParc UPI00000C504C
UniProtKB Acc Q9I689
UniProtKB ID Q9I689_PSEAE
UniRef100 UniRef100_Q9I689
UniRef50 UniRef50_Q4K4H4
UniRef90 UniRef90_Q9I689

Product

Feature Type CDS
Coding Frame 1
Product Name
probable ATP-dependent RNA helicase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 29.11
Kyte-Doolittle Hydrophobicity Value -0.819
Molecular Weight (kDa) 70.1
Isoelectric Point (pI) 10.65

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 640 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000419 (542 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0428

Human Homologs

Ensembl 97, assembly GRCh38.p12
DEAD-box helicase 27 [Source:HGNC Symbol;Acc:HGNC:15837]
E-value: 2.5e-65
Percent Identity: 43.6

Human Homologs

Ensembl 97, assembly GRCh38.p12
DEAD-box helicase 27 [Source:HGNC Symbol;Acc:HGNC:15837]
E-value: 2.5e-65
Percent Identity: 43.6

Human Homologs

Ensembl 97, assembly GRCh38.p12
DEAD-box helicase 27 [Source:HGNC Symbol;Acc:HGNC:15837]
E-value: 2.5e-65
Percent Identity: 43.6

References

Structural analysis of the rhlE gene of Escherichia coli.
Ohmori H
Jpn. J. Genet. 1994 Feb;69(1):1-12
PubMed ID: 8037924
The protein family of RNA helicases.
Lüking A, Stahl U, Schmidt U
Crit. Rev. Biochem. Mol. Biol. 1998 ;33(4):259-96
PubMed ID: 9747670
Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation.
Raynal LC, Carpousis AJ
Mol. Microbiol. 1999 May;32(4):765-75
PubMed ID: 10361280