Pseudomonas aeruginosa PAO1, PA1195 (ddaH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1195
Name
ddaH
Replicon chromosome
Genomic location 1296334 - 1297098 (- strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_249886.1
GI 15596392
Affymetrix PA1195_at
Entrez 883112
GenBank AAG04584.1
INSDC AAG04584.1
NCBI Locus Tag PA1195
protein_id(GenBank) gb|AAG04584.1|AE004549_11|gnl|PseudoCAP|PA1195
TIGR NTL03PA01196
UniParc UPI0000033BA5
UniProtKB Acc Q9I4E3
UniProtKB ID DDAH_PSEAE
UniRef100 UniRef100_Q9I4E3
UniRef50 UniRef50_Q9I4E3
UniRef90 UniRef90_Q9I4E3

Product

Feature Type CDS
Coding Frame 1
Product Name
dimethylarginine dimethylaminohydrolase DdaH
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -6.46
Kyte-Doolittle Hydrophobicity Value -0.229
Molecular Weight (kDa) 28.5
Isoelectric Point (pI) 5.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3RHY HYDROLASE 04/12/11 Crystal structure of the dimethylarginine dimethylaminohydrolase adduct with 4-chloro-2-hydroxymethylpyridine PSEUDOMONAS AERUGINOSA 2.18 X-RAY DIFFRACTION 100.0
1H70 HYDROLASE 06/30/01 DDAH FROM PSEUDOMONAS AERUGINOSA. C249S MUTANT COMPLEXED WITH CITRULLINE PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 99.6
3BPB HYDROLASE 12/18/07 Crystal structure of the dimethylarginine dimethylaminohydrolase H162G adduct with S-methyl-L-thiocitrulline PSEUDOMONAS AERUGINOSA 2.81 X-RAY DIFFRACTION 99.6

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 96 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001153 (303 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1195
Search term: ddaH

Human Homologs

Ensembl 86, assembly GRCh38.p7
dimethylarginine dimethylaminohydrolase 1 [Source:HGNC Symbol;Acc:HGNC:2715]
E-value: 1.0e-24
Percent Identity: 30.8

Transposon Insertions in PAO1

Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 8261
Genomic Position 1296436
Position in ORF 103(765)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 8261
Genomic Position 1296436
Position in ORF 103(765)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 1720
Genomic Position 1296585
Position in ORF 252(765)
Reference 14617778

Transposon Insertions in Orthologs

References

Cloning and sequencing of cDNA encoding NG,NG-dimethylarginine dimethylaminohydrolase from rat kidney.
Kimoto M, Sasakawa T, Tsuji H, Miyatake S, Oka T, Nio N, Ogawa T
Biochim. Biophys. Acta 1997 Jan;1337(1):6-10
PubMed ID: 9003431
DdaR (PA1196) Regulates Expression of Dimethylarginine Dimethylaminohydrolase for the Metabolism of Methylarginines in Pseudomonas aeruginosa PAO1.
Lundgren BR, Bailey FJ, Moley G, Nomura CT
J. Bacteriol. 2017 Apr;199(8):
PubMed ID: 28167521