Pseudomonas aeruginosa PAO1, PA0018 (fmt)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0018
Name
fmt
Replicon chromosome
Genomic location 20068 - 21012 (- strand)

Cross-References

RefSeq NP_248708.1
GI 15595216
Affymetrix PA0018_fmt_at
Entrez 879258
GenBank AAG03408.1
NCBI Locus Tag PA0018
protein_id(GenBank) gb|AAG03408.1|AE004441_9|gnl|PseudoCAP|PA0018
TIGR NTL03PA00019
UniProtKB Acc O85732
UniProtKB ID FMT_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
methionyl-tRNA formyltransferase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.17
Kyte-Doolittle Hydrophobicity Value 0.068
Molecular Weight (kDa) 33.0
Isoelectric Point (pI) 6.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5UAI TRANSFERASE 12/19/16 Crystal structure of Methionyl-tRNA formyltransferase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PR 2.75 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 600 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000018 (560 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0018
Search term: fmt

References

Disruption of the gene for Met-tRNA(fMet) formyltransferase severely impairs growth of Escherichia coli.
Guillon JM, Mechulam Y, Schmitter JM, Blanquet S, Fayat G
J. Bacteriol. 1992 Jul;174(13):4294-301
PubMed ID: 1624424
The Escherichia coli fmt gene, encoding methionyl-tRNA(fMet) formyltransferase, escapes metabolic control.
Meinnel T, Guillon JM, Mechulam Y, Blanquet S
J. Bacteriol. 1993 Feb;175(4):993-1000
PubMed ID: 8432722
Structure of crystalline Escherichia coli methionyl-tRNA(f)Met formyltransferase: comparison with glycinamide ribonucleotide formyltransferase.
Schmitt E, Blanquet S, Mechulam Y
EMBO J. 1996 Sep;15(17):4749-58
PubMed ID: 8887566