Pseudomonas aeruginosa PAO1, PA0796 (prpB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0796
Name
prpB
Synonym: bcpA
Replicon chromosome
Genomic location 874206 - 875102 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249487.1
GI 15595993
Affymetrix PA0796_prpB_at
Entrez 882026
GenBank AAG04185.1
INSDC AAG04185.1
NCBI Locus Tag PA0796
protein_id(GenBank) gb|AAG04185.1|AE004515_1|gnl|PseudoCAP|PA0796
TIGR NTL03PA00797
UniParc UPI00000C5159
UniProtKB Acc Q9I5E2
UniProtKB ID Q9I5E2_PSEAE
UniRef100 UniRef100_W0YNY4
UniRef50 UniRef50_Q56062
UniRef90 UniRef90_W0YNY4

Product

Feature Type CDS
Coding Frame 1
Product Name
carboxyphosphonoenolpyruvate phosphonomutase
Product Name Confidence: Class 2
Synonyms CPEP phosphonomutase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.92
Kyte-Doolittle Hydrophobicity Value 0.032
Molecular Weight (kDa) 32.1
Isoelectric Point (pI) 5.20

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6T4V LYASE 10/15/19 Crystal structure of 2-methylisocitrate lyase (PrpB) from Pseudomonas aeruginosa in apo form. Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.807 X-RAY DIFFRACTION 100.0
6T5M LYASE 10/16/19 Crystal structure of 2-methylisocitrate lyase (PrpB) from Pseudomonas aeruginosa in complex with Mg(II)-pyruvate. Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.76 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 343 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000769 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0796
Search term: prpB

Human Homologs

References

Cloning, overexpression and mechanistic studies of carboxyphosphonoenolpyruvate mutase from Streptomyces hygroscopicus.
Pollack SJ, Freeman S, Pompliano DL, Knowles JR
Eur J Biochem 1992 Oct 15;209(2):735-43
PubMed ID: 1330557
Propionate catabolism in Salmonella typhimurium LT2: two divergently transcribed units comprise the prp locus at 8.5 centisomes, prpR encodes a member of the sigma-54 family of activators, and the prpBCDE genes constitute an operon.
Horswill AR, Escalante-Semerena JC
J Bacteriol 1997 Feb;179(3):928-40
PubMed ID: 9006051