Pseudomonas aeruginosa PAO1, PA1529 (lig)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1529
Name
lig
Synonym: dnaL
Synonym: ligA
Replicon chromosome
Genomic location 1666025 - 1668409 (+ strand)

Cross-References

RefSeq NP_250220.1
GI 15596726
Affymetrix PA1529_lig_at
Entrez 879269
GenBank AAG04918.1
INSDC AAG04918.1
NCBI Locus Tag PA1529
protein_id(GenBank) gb|AAG04918.1|AE004581_5|gnl|PseudoCAP|PA1529
TIGR NTL03PA01530
UniParc UPI00000C53C6
UniProtKB Acc Q9I3I4
UniProtKB ID DNLJ_PSEAE
UniRef100 UniRef100_B7UVJ3
UniRef50 UniRef50_Q9KTD1
UniRef90 UniRef90_B7UVJ3

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA ligase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -19.07
Kyte-Doolittle Hydrophobicity Value -0.177
Molecular Weight (kDa) 86.8
Isoelectric Point (pI) 5.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 599 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003829 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1529
Search term: lig
Search term: DNA ligase

Human Homologs

References

Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli.
Ishino Y, Shinagawa H, Makino K, Tsunasawa S, Sakiyama F, Nakata A
Mol Gen Genet 1986 Jul;204(1):1-7
PubMed ID: 3018436
Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease.
Heitman J, Zinder ND, Model P
Proc Natl Acad Sci U S A 1989 Apr;86(7):2281-5
PubMed ID: 2648397