Pseudomonas aeruginosa PAO1, PA1543 (apt)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1543
Name
apt
Replicon chromosome
Genomic location 1680437 - 1680985 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250234.1
GI 15596740
Affymetrix PA1543_apt_at
Entrez 879394
GenBank AAG04932.1
INSDC CAA40907.1
NCBI Locus Tag PA1543
protein_id(GenBank) gb|AAG04932.1|AE004582_10|gnl|PseudoCAP|PA1543
TIGR NTL03PA01544
UniParc UPI0000125CE5
UniProtKB Acc Q04633
UniProtKB ID APT_PSEAE
UniRef100 UniRef100_B7UVH9
UniRef50 UniRef50_Q4KFF5
UniRef90 UniRef90_B7UVH9

Product

Feature Type CDS
Coding Frame 1
Product Name
adenine phosphoribosyltransferase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.55
Kyte-Doolittle Hydrophobicity Value 0.248
Molecular Weight (kDa) 19.8
Isoelectric Point (pI) 4.58

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 483 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003817 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1543
Search term: apt

Human Homologs

Ensembl 110, assembly GRCh38.p14
adenine phosphoribosyltransferase [Source:HGNC Symbol;Acc:HGNC:626]
E-value: 2.6e-30
Percent Identity: 41.4

References

Nucleotide sequence and deduced amino acid sequence of Escherichia coli adenine phosphoribosyltransferase and comparison with other analogous enzymes.
Hershey HV, Taylor MW
Gene 1986;43(3):287-93
PubMed ID: 3527873
Anaerobic growth and cyanide synthesis of Pseudomonas aeruginosa depend on anr, a regulatory gene homologous with fnr of Escherichia coli.
Zimmermann A, Reimmann C, Galimand M, Haas D
Mol Microbiol 1991 Jun;5(6):1483-90
PubMed ID: 1787798