Pseudomonas aeruginosa PAO1, PA1580 (gltA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1580
Name
gltA
Synonym: cisY
Replicon chromosome
Genomic location 1719109 - 1720395 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250271.1
GI 15596777
Affymetrix PA1580_gltA_at
Entrez 882117
GenBank AAG04969.1
INSDC AAG04969.1
NCBI Locus Tag PA1580
protein_id(GenBank) gb|AAG04969.1|AE004586_7|gnl|PseudoCAP|PA1580
TIGR NTL03PA01581
UniParc UPI0000127A1B
UniProtKB Acc P14165
UniProtKB ID CISY_PSEAE
UniRef100 UniRef100_P14165
UniRef50 UniRef50_P14165
UniRef90 UniRef90_P14165

Product

Feature Type CDS
Coding Frame 1
Product Name
citrate synthase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.33
Kyte-Doolittle Hydrophobicity Value -0.206
Molecular Weight (kDa) 47.7
Isoelectric Point (pI) 6.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6ZU0 TRANSFERASE 07/21/20 Crystal structure of citrate synthase (GltA) from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.397 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 552 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003783 (540 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1580
Search term: gltA
Search term: citrate synthase

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning, sequencing, and expression of the gene for NADH-sensitive citrate synthase of Pseudomonas aeruginosa.
Donald LJ, Molgat GF, Duckworth HW
J Bacteriol 1989 Oct;171(10):5542-50
PubMed ID: 2507528
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994