Pseudomonas aeruginosa PAO1, PA1585 (sucA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1585
Name
sucA
Replicon chromosome
Genomic location 1724244 - 1727075 (+ strand)

Cross-References

RefSeq NP_250276.1
GI 15596782
Affymetrix PA1585_sucA_at
Entrez 881879
GenBank AAG04974.1
INSDC AAG04974.1
NCBI Locus Tag PA1585
protein_id(GenBank) gb|AAG04974.1|AE004586_12|gnl|PseudoCAP|PA1585
TIGR NTL03PA01586
UniParc UPI00000C53F4
UniProtKB Acc Q9I3D3
UniProtKB ID Q9I3D3_PSEAE
UniRef100 UniRef100_Q9I3D3
UniRef50 UniRef50_Q4KFY9
UniRef90 UniRef90_A6V7K8

Product

Feature Type CDS
Coding Frame 1
Product Name
2-oxoglutarate dehydrogenase (E1 subunit)
Product Name Confidence: Class 2
Synonyms alpha-ketoglutarate dehydrogenase
Evidence for Translation
Detected using LC-MS/MS (105.88 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.83
Kyte-Doolittle Hydrophobicity Value -0.378
Molecular Weight (kDa) 105.9
Isoelectric Point (pI) 6.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 367 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003778 (538 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1585
Search term: sucA

Human Homologs

Ensembl 110, assembly GRCh38.p14
oxoglutarate dehydrogenase [Source:HGNC Symbol;Acc:HGNC:8124]
E-value: 1.0e-194
Percent Identity: 41.5
Ensembl 110, assembly GRCh38.p14
oxoglutarate dehydrogenase [Source:HGNC Symbol;Acc:HGNC:8124]
E-value: 1.0e-194
Percent Identity: 41.5

References

Cloning and expression of the succinyl-CoA synthetase genes of Escherichia coli K12.
Buck D, Spencer ME, Guest JR
J Gen Microbiol 1986 Jun;132(6):1753-62
PubMed ID: 3543212
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344