Pseudomonas aeruginosa PAO1, PA1609 (fabB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1609
Name
fabB
Replicon chromosome
Genomic location 1751674 - 1752891 (- strand)

Cross-References

RefSeq NP_250300.1
GI 15596806
Affymetrix PA1609_fabB_at
Entrez 881925
GenBank AAG04998.1
INSDC AAG04998.1
NCBI Locus Tag PA1609
protein_id(GenBank) gb|AAG04998.1|AE004589_1|gnl|PseudoCAP|PA1609
TIGR NTL03PA01610
UniParc UPI00000C5407
UniProtKB Acc Q9I3B2
UniProtKB ID Q9I3B2_PSEAE
UniRef100 UniRef100_Q9I3B2
UniRef50 UniRef50_Q02K94
UniRef90 UniRef90_Q02K94

Product

Feature Type CDS
Coding Frame 1
Product Name
beta-ketoacyl-ACP synthase I
Product Name Confidence: Class 1
Synonyms FabB
KAS I
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -7.52
Kyte-Doolittle Hydrophobicity Value -0.087
Molecular Weight (kDa) 42.8
Isoelectric Point (pI) 5.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7PPS TRANSFERASE 09/15/21 apo FabB from Pseudomonas aeruginosa with single point mutation C161A Pseudomonas aeruginosa PA14 1.3 X-RAY DIFFRACTION 99.5

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 572 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003761 (538 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1609
Search term: fabB

Human Homologs

Ensembl 110, assembly GRCh38.p14
3-oxoacyl-ACP synthase, mitochondrial [Source:HGNC Symbol;Acc:HGNC:26063]
E-value: 4.6e-56
Percent Identity: 34.8

References

beta-Ketoacyl-ACP synthase I of Escherichia coli: nucleotide sequence of the fabB gene and identification of the cerulenin binding residue.
Kauppinen S, Siggaard-Andersen M, von Wettstein-Knowles P
Carlsberg Res Commun 1988;53(6):357-70
PubMed ID: 3076376
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994