Pseudomonas aeruginosa PAO1, PA1674 (folE2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1674
Name
folE2
Replicon chromosome
Genomic location 1825495 - 1826040 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250365.1
GI 15596871
Affymetrix PA1674_folE2_at
Entrez 879308
GenBank AAG05063.1
INSDC AAG05063.1
NCBI Locus Tag PA1674
protein_id(GenBank) gb|AAG05063.1|AE004595_2|gnl|PseudoCAP|PA1674
TIGR NTL03PA01675
UniParc UPI000012B278
UniProtKB Acc Q9I351
UniProtKB ID GCH12_PSEAE
UniRef100 UniRef100_Q9I351
UniRef50 UniRef50_Q2JPT8
UniRef90 UniRef90_Q9I351

Product

Feature Type CDS
Coding Frame 1
Product Name
GTP cyclohydrolase I precursor
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (20.24 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.10
Kyte-Doolittle Hydrophobicity Value -0.043
Molecular Weight (kDa) 20.2
Isoelectric Point (pI) 7.56

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 471 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003716 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1674
Search term: folE2

Human Homologs

Ensembl 110, assembly GRCh38.p14
GTP cyclohydrolase 1 [Source:HGNC Symbol;Acc:HGNC:4193]
E-value: 1.2e-56
Percent Identity: 56.7
Ensembl 110, assembly GRCh38.p14
GTP cyclohydrolase 1 [Source:HGNC Symbol;Acc:HGNC:4193]
E-value: 1.2e-56
Percent Identity: 56.7

References

Cloning and sequencing of cDNA encoding rat GTP cyclohydrolase I. The first enzyme of the tetrahydrobiopterin biosynthetic pathway.
Hatakeyama K, Inoue Y, Harada T, Kagamiyama H
J Biol Chem 1991 Jan 15;266(2):765-9
PubMed ID: 1985963
Allosteric characteristics of GTP cyclohydrolase I from Escherichia coli.
Schoedon G, Redweik U, Frank G, Cotton RG, Blau N
Eur J Biochem 1992 Dec 1;210(2):561-8
PubMed ID: 1459137
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344