Pseudomonas aeruginosa PAO1, PA1804 (hupB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1804
Name
hupB
Replicon chromosome
Genomic location 1958759 - 1959031 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_250495.1
GI 15597001
Affymetrix PA1804_hupB_at
Entrez 878308
GenBank AAG05193.1
INSDC AAG05193.1
NCBI Locus Tag PA1804
protein_id(GenBank) gb|AAG05193.1|AE004606_7|gnl|PseudoCAP|PA1804
TIGR NTL03PA01805
UniParc UPI0000128EF9
UniProtKB Acc P05384
UniProtKB ID DBHB_PSEAE
UniRef100 UniRef100_P05384
UniRef50 UniRef50_P96045
UniRef90 UniRef90_P05384

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA-binding protein HU
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis, mass spectral analysis and protein mass mapping (PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (9.09 kDa, PMID:21751344)
Charge (pH 7) 5.98
Kyte-Doolittle Hydrophobicity Value 0.177
Molecular Weight (kDa) 9.1
Isoelectric Point (pI) 10.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 569 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003593 (526 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1804
Search term: hupB

Human Homologs

References

The Pseudomonas aeruginosa global regulator MvaT specifically binds to the ptxS upstream region and enhances ptxS expression.
Westfall LW, Luna AM, Francisco MS, Diggle SP, Worrall KE, Williams P, Cámara M, Hamood AN
Microbiology (Reading) 2004 Nov;150(Pt 11):3797-3806
PubMed ID: 15528665
A single DNA-binding protein from Pseudomonas aeruginosa homologous to proteins NS1 and NS2 (HU proteins) of Escherichia coli and other bacteria.
Hawkins AR, Wootton JC
FEBS Lett 1981 Aug 3;130(2):275-8
PubMed ID: 6793399
HU-1 mutants of Escherichia coli deficient in DNA binding.
Goshima N, Kohno K, Imamoto F, Kano Y
Gene 1990 Nov 30;96(1):141-5
PubMed ID: 2265752
Cloning and sequence analyses of the genes coding for the integration host factor (IHF) and HU proteins of Pseudomonas aeruginosa.
Delic-Attree I, Toussaint B, Vignais PM
Gene 1995 Feb 27;154(1):61-4
PubMed ID: 7867950
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344