Pseudomonas aeruginosa PAO1, PA1820 (nhaB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1820
Name
nhaB
Replicon chromosome
Genomic location 1978439 - 1979941 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250511.1
GI 15597017
Affymetrix PA1820_nhaB_at
Entrez 877826
GenBank AAG05209.1
INSDC AAG05209.1
NCBI Locus Tag PA1820
protein_id(GenBank) gb|AAG05209.1|AE004608_8|gnl|PseudoCAP|PA1820
TIGR NTL03PA01821
UniParc UPI00000C54B1
UniProtKB Acc Q9I2S5
UniProtKB ID NHAB_PSEAE
UniRef100 UniRef100_B7VB57
UniRef50 UniRef50_Q56577
UniRef90 UniRef90_B7VB57

Product

Feature Type CDS
Coding Frame 1
Product Name
sodium/proton antiporter NhaB
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.48
Kyte-Doolittle Hydrophobicity Value 0.806
Molecular Weight (kDa) 54.2
Isoelectric Point (pI) 7.48

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 65 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003578 (400 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1820
Search term: nhaB

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning, sequencing, and expression of the nhaB gene, encoding a Na+/H+ antiporter in Escherichia coli.
Pinner E, Padan E, Schuldiner S
J Biol Chem 1992 Jun 5;267(16):11064-8
PubMed ID: 1317851
Cloning and sequencing of the gene for Na+/H+ antiporter of Vibrio parahaemolyticus.
Nozaki K, Inaba K, Kuroda T, Tsuda M, Tsuchiya T
Biochem Biophys Res Commun 1996 May 24;222(3):774-9
PubMed ID: 8651921