Pseudomonas aeruginosa PAO1, PA1863 (modA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA1863
Name	modA
Replicon	chromosome
Genomic location	2022411 - 2023166 (- strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_250554.1
GI	15597060
Affymetrix	PA1863_modA_at
Entrez	877661
GenBank	AAG05252.1
INSDC	AAG05252.1
NCBI Locus Tag	PA1863
protein_id(GenBank)	gb|AAG05252.1|AE004612_6|gnl|PseudoCAP|PA1863
TIGR	NTL03PA01864
UniParc	UPI00000C54DB
UniProtKB Acc	Q9I2N2
UniProtKB ID	Q9I2N2_PSEAE
UniRef100	UniRef100_Q9I2N2
UniRef50	UniRef50_P37734
UniRef90	UniRef90_Q9I2N2

Product

Feature Type	CDS
Coding Frame	1
Product Name	molybdate-binding periplasmic protein precursor ModA Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Detected by MALDI-TOF/TOF (PMID:19333994). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-0.29
Kyte-Doolittle Hydrophobicity Value	0.070
Molecular Weight (kDa)	26.4
Isoelectric Point (pI)	6.81

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 3 20472543 Periplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7T51	METAL BINDING PROTEIN	12/11/21	Crystal structure of the molybdate-binding periplasmic protein ModA from the bacteria Pseudomonsa aeruginosa in molybdate-bound form	Pseudomonas aeruginosa PA1	2.5	X-RAY DIFFRACTION	99.1
7T4Z	METAL BINDING PROTEIN	12/10/21	Crystal structure of the molybdate-binding periplasmic protein ModA from the bacteria Pseudomonsa aeruginosa in ligand-free form	Pseudomonas aeruginosa PA1	1.78	X-RAY DIFFRACTION	99.1
7T5A	METAL BINDING PROTEIN	12/11/21	Crystal structure of the molybdate-binding periplasmic protein ModA from the bacteria Pseudomonsa aeruginosa in tungstate-bound form	Pseudomonas aeruginosa PA1	2.16	X-RAY DIFFRACTION	99.1
7T50	METAL BINDING PROTEIN	12/11/21	Crystal structure of the molybdate-binding periplasmic protein ModA from the bacteria Pseudomonsa aeruginosa in chromate-bound form	Pseudomonas aeruginosa PA1	1.9	X-RAY DIFFRACTION	99.1

Virulence Evidence

Source 1
Database	Victors : 4202
Category	Not available / Not known
Evidence	ECO:0000315 mutant phenotype evidence used in manual assertion
Host Organism	Rattus norvegicus
Notes
Infection model	Rat agar bead model of chronic lung infection
Reference	14641575

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 334 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG003540 (528 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA1863 Search term: modA Search term: molybdate-binding periplasmic protein precursor ModA

Human Homologs

References

Characterization of genes involved in molybdenum transport in Azotobacter vinelandii. Luque F, Mitchenall LA, Chapman M, Christine R, Pau RN Mol Microbiol 1993 Feb;7(3):447-59 PubMed ID: 8384683

Genetic analysis of the modABCD (molybdate transport) operon of Escherichia coli. Maupin-Furlow JA, Rosentel JK, Lee JH, Deppenmeier U, Gunsalus RP, Shanmugam KT J Bacteriol 1995 Sep;177(17):4851-6 PubMed ID: 7665460

Properties of the periplasmic ModA molybdate-binding protein of Escherichia coli. Rech S, Wolin C, Gunsalus RP J Biol Chem 1996 Feb 2;271(5):2557-62 PubMed ID: 8576221

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994