Pseudomonas aeruginosa PAO1, PA2250 (lpdV)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2250
Name
lpdV
Replicon chromosome
Genomic location 2476785 - 2478179 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250940.1
GI 15597446
Affymetrix PA2250_lpdV_at
Entrez 878470
GenBank AAG05638.1
INSDC AAG05638.1
NCBI Locus Tag PA2250
protein_id(GenBank) gb|AAG05638.1|AE004650_9|gnl|PseudoCAP|PA2250
TIGR NTL03PA02250
UniParc UPI00000C562D
UniProtKB Acc Q9I1L9
UniProtKB ID DLDH1_PSEAE
UniRef100 UniRef100_Q9I1L9
UniRef50 UniRef50_P09063
UniRef90 UniRef90_Q9I1L9

Product

Feature Type CDS
Coding Frame 1
Product Name
lipoamide dehydrogenase-Val
Product Name Confidence: Class 2
Synonyms Lpd-Val
lipoamide dehydrogenase (E3 subunit)
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.52
Kyte-Doolittle Hydrophobicity Value 0.185
Molecular Weight (kDa) 48.6
Isoelectric Point (pI) 6.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 646 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003239 (459 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2250
Search term: lpdV

Human Homologs

Ensembl 110, assembly GRCh38.p14
dihydrolipoamide dehydrogenase [Source:HGNC Symbol;Acc:HGNC:2898]
E-value: 1.4e-77
Percent Identity: 37.3
Ensembl 110, assembly GRCh38.p14
dihydrolipoamide dehydrogenase [Source:HGNC Symbol;Acc:HGNC:2898]
E-value: 1.4e-77
Percent Identity: 37.3

References

Sequence analysis of the lpdV gene for lipoamide dehydrogenase of branched-chain-oxoacid dehydrogenase of Pseudomonas putida.
Burns G, Brown T, Hatter K, Sokatch JR
Eur J Biochem 1989 Jan 15;179(1):61-9
PubMed ID: 2917566
The refined crystal structure of Pseudomonas putida lipoamide dehydrogenase complexed with NAD+ at 2.45 A resolution.
Mattevi A, Obmolova G, Sokatch JR, Betzel C, Hol WG
Proteins 1992 Aug;13(4):336-51
PubMed ID: 1325638