Pseudomonas aeruginosa PAO1, PA2290 (gcd)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2290
Name
gcd
Replicon chromosome
Genomic location 2518693 - 2521104 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250980.1
GI 15597486
Affymetrix PA2290_gcd_at
Entrez 877639
GenBank AAG05678.1
INSDC AAG05678.1
NCBI Locus Tag PA2290
protein_id(GenBank) gb|AAG05678.1|AE004654_9|gnl|PseudoCAP|PA2290
TIGR NTL03PA02290
UniParc UPI00000C564C
UniProtKB Acc Q9I1I5
UniProtKB ID Q9I1I5_PSEAE
UniRef100 UniRef100_Q9I1I5
UniRef50 UniRef50_Q48EX4
UniRef90 UniRef90_Q9I1I5

Product

Feature Type CDS
Coding Frame 1
Product Name
glucose dehydrogenase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (86.22 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.76
Kyte-Doolittle Hydrophobicity Value -0.098
Molecular Weight (kDa) 86.2
Isoelectric Point (pI) 6.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 146 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003207 (517 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2290
Search term: gcd

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Characterization of the gcd gene from Escherichia coli K-12 W3110 and regulation of its expression.
Yamada M, Asaoka S, Saier MH Jr, Yamada Y
J Bacteriol 1993 Jan;175(2):568-71
PubMed ID: 8419307
Topological analysis of quinoprotein glucose dehydrogenase in Escherichia coli and its ubiquinone-binding site.
Yamada M, Sumi K, Matsushita K, Adachi O, Yamada Y
J Biol Chem 1993 Jun 15;268(17):12812-7
PubMed ID: 8509415
Structure of the quinoprotein glucose dehydrogenase of Escherichia coli modelled on that of methanol dehydrogenase from Methylobacterium extorquens.
Cozier GE, Anthony C
Biochem J 1995 Dec 15;312 ( Pt 3)(Pt 3):679-85
PubMed ID: 8554505
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344