Pseudomonas aeruginosa PAO1, PA2398 (fpvA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2398
Name
fpvA
Replicon chromosome
Genomic location 2655187 - 2657634 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq NP_251088.1
GI 15597594
Affymetrix PA2398_fpvA_at
Entrez 878605
GenBank AAG05786.1
NCBI Locus Tag PA2398
protein_id(GenBank) gb|AAG05786.1|AE004666_2|gnl|PseudoCAP|PA2398
TIGR NTL03PA02398
UniProtKB Acc P48632
UniProtKB ID FPVA_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
ferripyoverdine receptor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.4, molecular weight:91.2, PMID:21674802)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -14.61
Kyte-Doolittle Hydrophobicity Value -0.557
Molecular Weight (kDa) 91.2
Isoelectric Point (pI) 5.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2IAH MEMBRANE PROTEIN 09/08/06 Crystal structure of the ferripyoverdine receptor of the outer membrane of Pseudomonas aeruginosa bound to ferripyoverdine. Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 2.73 X-RAY DIFFRACTION 100.0
1XKH MEMBRANE PROTEIN 09/29/04 Pyoverdine outer membrane receptor FpvA from Pseudomonas aeruginosa PAO1 bound to pyoverdine Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.6 X-RAY DIFFRACTION 100.0
2W6U MEMBRANE PROTEIN 12/19/08 Structures of P. aeruginosa FpvA bound to heterologous pyoverdines: FpvA-Pvd(G173)-Fe complex PSEUDOMONAS AERUGINOSA; PSEUDOMONAS FLUORESCENS 3 X-RAY DIFFRACTION 100.0
2W77 RECEPTOR 12/20/08 Structures of P. aeruginosa FpvA bound to heterologous pyoverdines: FpvA-Pvd(Pfl18.1)-Fe complex PSEUDOMONAS AERUGINOSA; PSEUDOMONAS FLUORESCENS 2.9 X-RAY DIFFRACTION 100.0
2W76 RECEPTOR 12/20/08 Structures of P. aeruginosa FpvA bound to heterologous pyoverdines: FpvA-Pvd(Pa6)-Fe complex PSEUDOMONAS AERUGINOSA 2.8 X-RAY DIFFRACTION 100.0
2O5P TRANSPORT PROTEIN 12/06/06 Crystal structure of the full length ferric pyoverdine outer membrane receptor FpvA of Pseudomonas aeruginosa in its apo form Pseudomonas aeruginosa 2.77 X-RAY DIFFRACTION 100.0
2W75 RECEPTOR 12/20/08 Structures of P. aeruginosa FpvA bound to heterologous pyoverdines: Apo-FpvA PSEUDOMONAS AERUGINOSA 2.9 X-RAY DIFFRACTION 100.0
5ODW PROTEIN TRANSPORT 07/06/17 Structure of the FpvAI-pyocin S2 complex Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.8 X-RAY DIFFRACTION 100.0
2W16 MEMBRANE PROTEIN 10/14/08 Structures of FpvA bound to heterologous pyoverdines PSEUDOMONAS AERUGINOSA 2.71 X-RAY DIFFRACTION 100.0
2W6T MEMBRANE PROTEIN 12/19/08 Structures of P. aeruginosa FpvA bound to heterologous pyoverdines: FpvA-Pvd(DSM50106)-Fe complex PSEUDOMONAS AERUGINOSA; PSEUDOMONAS FLUORESCENS 2.9 X-RAY DIFFRACTION 100.0
2W78 RECEPTOR 12/20/08 Structures of P. aeruginosa FpvA bound to heterologous pyoverdines: FpvA-Pvd(ATCC13535)-Fe complex PSEUDOMONAS AERUGINOSA 3 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
3,6,9,12,15-PENTAOXATRICOSAN-1-OL DB08249 DrugBank 5.1.4 P48632 Ferripyoverdine receptor 100.0 815 0.0e+00

Virulence Evidence

Source 1
Database VFDB : VF0094
Category Pyoverdine receptors
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 271 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001486 (1037 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2398
Search term: fpvA

Human Homologs

References

Cloning and nucleotide sequence analysis of the ferripyoverdine receptor gene fpvA of Pseudomonas aeruginosa.
Poole K, Neshat S, Krebes K, Heinrichs DE
J Bacteriol 1993 Aug;175(15):4597-604
PubMed ID: 8335619
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802