Pseudomonas aeruginosa PAO1, PA2629 (purB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2629
Name
purB
Replicon chromosome
Genomic location 2972699 - 2974069 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_251319.1
GI 15597825
Affymetrix PA2629_purB_at
Entrez 882336
GenBank AAG06017.1
INSDC AAG06017.1
NCBI Locus Tag PA2629
protein_id(GenBank) gb|AAG06017.1|AE004691_8|gnl|PseudoCAP|PA2629
TIGR NTL03PA02629
UniParc UPI00000C5776
UniProtKB Acc Q9I0K9
UniProtKB ID PUR8_PSEAE
UniRef100 UniRef100_Q9I0K9
UniRef50 UniRef50_Q9I0K9
UniRef90 UniRef90_Q9I0K9

Product

Feature Type CDS
Coding Frame 1
Product Name
adenylosuccinate lyase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.39
Kyte-Doolittle Hydrophobicity Value -0.116
Molecular Weight (kDa) 50.5
Isoelectric Point (pI) 5.66

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7T24 LYASE 12/03/21 Crystal Structure of Adenylosuccinate lyase (ASL) from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.45 X-RAY DIFFRACTION 100.0
7T29 LYASE 12/03/21 Crystal Structure of Adenylosuccinate lyase (ASL) from Pseudomonas aeruginosa complexed with AMP Pseudomonas aeruginosa 1.5 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 602 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002924 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2629
Search term: purB

Human Homologs

References

A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.
Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T
DNA Res 1996 Jun 30;3(3):137-55
PubMed ID: 8905232