Pseudomonas aeruginosa PAO1, PA2644 (nuoI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2644
Name
nuoI
Replicon chromosome
Genomic location 2991442 - 2991990 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251334.1
GI 15597840
Affymetrix PA2644_nuoI_at
DNASU PaCD00007140
Entrez 882353
GenBank AAG06032.1
INSDC AAG06032.1
NCBI Locus Tag PA2644
protein_id(GenBank) gb|AAG06032.1|AE004693_3|gnl|PseudoCAP|PA2644
TIGR NTL03PA02644
UniParc UPI00000D429E
UniProtKB Acc Q9I0J4
UniProtKB ID NUOI_PSEAE
UniRef100 UniRef100_A6V4E1
UniRef50 UniRef50_Q8RQ74
UniRef90 UniRef90_Q8RQ74

Product

Feature Type CDS
Coding Frame 1
Product Name
NADH Dehydrogenase I chain I
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.56
Kyte-Doolittle Hydrophobicity Value -0.238
Molecular Weight (kDa) 20.6
Isoelectric Point (pI) 6.72

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7P7K PROTON TRANSPORT 07/19/21 Complex I from E. coli, DDM/LMNG-purified, with DQ, Resting state Escherichia coli BL21(DE3) 3.1 ELECTRON MICROSCOPY 91.2
7P62 MEMBRANE PROTEIN 07/15/21 Complex I from E. coli, DDM-purified, Apo, Resting state Escherichia coli BL21(DE3) 3.6 ELECTRON MICROSCOPY 91.0
7P61 PROTON TRANSPORT 07/15/21 Complex I from E. coli, DDM-purified, with NADH, Resting state Escherichia coli BL21(DE3) 3.2 ELECTRON MICROSCOPY 91.2
7P69 PROTON TRANSPORT 07/15/21 Complex I from E. coli, DDM/LMNG-purified, under Turnover at pH 6, Resting state Escherichia coli BL21(DE3) 3.0 ELECTRON MICROSCOPY 91.0
7P7E PROTON TRANSPORT 07/19/21 Complex I from E. coli, DDM/LMNG-purified, Apo, Resting state Escherichia coli BL21(DE3) 2.7 ELECTRON MICROSCOPY 91.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 508 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002910 (518 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2644
Search term: nuoI

Human Homologs

References

The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I.
Weidner U, Geier S, Ptock A, Friedrich T, Leif H, Weiss H
J Mol Biol 1993 Sep 5;233(1):109-22
PubMed ID: 7690854