Pseudomonas aeruginosa PAO1, PA2873 (tgpA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2873
Name
tgpA
Replicon chromosome
Genomic location 3225381 - 3227387 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs
CommentTgpA plays a critical role in the viability of P. aeruginosa.

Cross-References

RefSeq NP_251563.1
GI 15598069
Affymetrix PA2873_at
Entrez 882644
GenBank AAG06261.1
INSDC AAG06261.1
NCBI Locus Tag PA2873
protein_id(GenBank) gb|AAG06261.1|AE004713_10|gnl|PseudoCAP|PA2873
TIGR NTL03PA02873
UniParc UPI00000C5855
UniProtKB Acc Q9HZX3
UniProtKB ID TGPA_PSEAE
UniRef100 UniRef100_Q9HZX3
UniRef50 UniRef50_Q9HZX3
UniRef90 UniRef90_Q9HZX3

Product

Feature Type CDS
Coding Frame 1
Product Name
transglutaminase protein A, TgpA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 16.60
Kyte-Doolittle Hydrophobicity Value 0.015
Molecular Weight (kDa) 74.8
Isoelectric Point (pI) 10.11

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6G49 TRANSFERASE 03/27/18 Crystal structure of the periplasmic domain of TgpA from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 1.6 X-RAY DIFFRACTION 100.0
6G4H TRANSFERASE 03/27/18 Crystal structure of the periplasmic domain of TgpA from Pseudomonas aeruginosa bound to ethylmercury Pseudomonas aeruginosa PAO1 1.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 289 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002698 (1284 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2873
Search term: tgpA

Human Homologs

References

TgpA, a protein with a eukaryotic-like transglutaminase domain, plays a critical role in the viability of Pseudomonas aeruginosa.
Milani A, Vecchietti D, Rusmini R, Bertoni G
PLoS One 2012;7(11):e50323
PubMed ID: 23209712