Pseudomonas aeruginosa PAO1, PA3083 (pepN)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3083
Name
pepN
Replicon chromosome
Genomic location 3460380 - 3463037 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_251773.1
GI 15598279
Affymetrix PA3083_pepN_at
Entrez 882952
GenBank AAG06471.1
INSDC AAG06471.1
NCBI Locus Tag PA3083
protein_id(GenBank) gb|AAG06471.1|AE004732_5|gnl|PseudoCAP|PA3083
TIGR NTL03PA03083
UniParc UPI00000C590A
UniProtKB Acc Q9HZC5
UniProtKB ID Q9HZC5_PSEAE
UniRef100 UniRef100_Q9HZC5
UniRef50 UniRef50_A6V2Z3
UniRef90 UniRef90_A6V2Z3

Product

Feature Type CDS
Coding Frame 1
Product Name
aminopeptidase N
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -31.15
Kyte-Doolittle Hydrophobicity Value -0.339
Molecular Weight (kDa) 100.0
Isoelectric Point (pI) 4.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 397 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002509 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3083
Search term: pepN
Search term: aminopeptidase N

Human Homologs

Ensembl 110, assembly GRCh38.p14
leucyl and cystinyl aminopeptidase [Source:HGNC Symbol;Acc:HGNC:6656]
E-value: 1.4e-28
Percent Identity: 27.0
Ensembl 110, assembly GRCh38.p14
leucyl and cystinyl aminopeptidase [Source:HGNC Symbol;Acc:HGNC:6656]
E-value: 1.4e-28
Percent Identity: 27.0

References

Nucleotide sequence of the promoter and amino-terminal encoding region of the Escherichia coli pepN gene.
Bally M, Foglino M, Bruschi M, Murgier M, Lazdunski A
Eur J Biochem 1986 Mar 17;155(3):565-9
PubMed ID: 2869947
Sequence of the promoter and 5' coding region of pepN, and the amino-terminus of peptidase N from Escherichia coli K-12.
McCaman MT, Gabe JD
Mol Gen Genet 1986 Jul;204(1):148-52
PubMed ID: 3018440
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994