Pseudomonas aeruginosa PAO1, PA3382 (phnE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3382
Name
phnE
Replicon chromosome
Genomic location 3788232 - 3789026 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252072.1
GI 15598578
Affymetrix PA3382_phnE_at
DNASU PaCD00007485
Entrez 878143
GenBank AAG06770.1
INSDC AAG06770.1
NCBI Locus Tag PA3382
protein_id(GenBank) gb|AAG06770.1|AE004759_17|gnl|PseudoCAP|PA3382
TIGR NTL03PA03382
UniParc UPI00000C59E3
UniProtKB Acc Q9HYL9
UniProtKB ID Q9HYL9_PSEAE
UniRef100 UniRef100_W0YT28
UniRef50 UniRef50_P16683
UniRef90 UniRef90_W0YT28

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphonate transport protein PhnE
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Charge (pH 7) -0.10
Kyte-Doolittle Hydrophobicity Value 0.877
Molecular Weight (kDa) 28.4
Isoelectric Point (pI) 6.58

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 227 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002246 (1270 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3382
Search term: phnE

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B.
Chen CM, Ye QZ, Zhu ZM, Wanner BL, Walsh CT
J Biol Chem 1990 Mar 15;265(8):4461-71
PubMed ID: 2155230
Molecular genetic analysis of phosphite and hypophosphite oxidation by Pseudomonas stutzeri WM88.
Metcalf WW, Wolfe RS
J Bacteriol 1998 Nov;180(21):5547-58
PubMed ID: 9791102