Pseudomonas aeruginosa PAO1, PA3524 (gloA1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3524
Name
gloA1
Replicon chromosome
Genomic location 3944184 - 3944570 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252214.1
GI 15598720
Affymetrix PA3524_gloA1_at
DNASU PaCD00007551
Entrez 879791
GenBank AAG06912.1
INSDC AAG06912.1
NCBI Locus Tag PA3524
protein_id(GenBank) gb|AAG06912.1|AE004773_1|gnl|PseudoCAP|PA3524
TIGR NTL03PA03524
UniParc UPI00000C5A53
UniProtKB Acc Q9HY85
UniProtKB ID Q9HY85_PSEAE
UniRef100 UniRef100_Q9HY85
UniRef50 UniRef50_Q55595
UniRef90 UniRef90_Q9HY85

Product

Feature Type CDS
Coding Frame 1
Product Name
lactoylglutathione lyase
Product Name Confidence: Class 1
Synonyms S-D-lactoylglutathione methylglyoxal lyase
methylglyoxalase
glyoxalase I
ketone-aldehyde mutase
aldoketomutase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.08
Kyte-Doolittle Hydrophobicity Value -0.238
Molecular Weight (kDa) 14.3
Isoelectric Point (pI) 4.68

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 345 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002141 (841 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3524
Search term: gloA1

Human Homologs

Ensembl 110, assembly GRCh38.p14
glyoxalase I [Source:HGNC Symbol;Acc:HGNC:4323]
E-value: 1.1e-19
Percent Identity: 39.3

References

Overproduction and characterization of a dimeric non-zinc glyoxalase I from Escherichia coli: evidence for optimal activation by nickel ions.
Clugston SL, Barnard JF, Kinach R, Miedema D, Ruman R, Daub E, Honek JF
Biochemistry 1998 Jun 16;37(24):8754-63
PubMed ID: 9628737
Distinct classes of glyoxalase I: metal specificity of the Yersinia pestis, Pseudomonas aeruginosa and Neisseria meningitidis enzymes.
Sukdeo N, Clugston SL, Daub E, Honek JF
Biochem J 2004 Nov 15;384(Pt 1):111-7
PubMed ID: 15270717