Pseudomonas aeruginosa PAO1, PA3823 (tgt)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3823
Name
tgt
Replicon chromosome
Genomic location 4279389 - 4280507 (- strand)
Transposon Mutants 14 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252512.1
GI 15599018
Affymetrix PA3823_tgt_at
Entrez 879886
GenBank AAG07210.1
INSDC AAG07210.1
NCBI Locus Tag PA3823
protein_id(GenBank) gb|AAG07210.1|AE004799_16|gnl|PseudoCAP|PA3823
TIGR NTL03PA03823
UniParc UPI0000136CF4
UniProtKB Acc Q9HXH9
UniProtKB ID TGT_PSEAE
UniRef100 UniRef100_B7UVU4
UniRef50 UniRef50_Q5SLI7
UniRef90 UniRef90_B7UVU4

Product

Feature Type CDS
Coding Frame 1
Product Name
queuine tRNA-ribosyltransferase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.81
Kyte-Doolittle Hydrophobicity Value -0.215
Molecular Weight (kDa) 41.2
Isoelectric Point (pI) 6.88

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 614 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001843 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3823
Search term: tgt

Human Homologs

Ensembl 110, assembly GRCh38.p14
queuine tRNA-ribosyltransferase catalytic subunit 1 [Source:HGNC Symbol;Acc:HGNC:23797]
E-value: 2.1e-79
Percent Identity: 43.2

References

Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes.
Reuter K, Slany R, Ullrich F, Kersten H
J Bacteriol 1991 Apr;173(7):2256-64
PubMed ID: 1706703
Serine 90 is required for enzymic activity by tRNA-guanine transglycosylase from Escherichia coli.
Reuter K, Chong S, Ullrich F, Kersten H, Garcia GA
Biochemistry 1994 Jun 14;33(23):7041-6
PubMed ID: 8003468