Pseudomonas aeruginosa PAO1, PA3996 (lis)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3996
Name
lis
Synonym: lipA
Replicon chromosome
Genomic location 4476994 - 4477977 (- strand)

Cross-References

RefSeq NP_252685.1
GI 15599191
Affymetrix PA3996_lipA_at
DNASU PaCD00007745
Entrez 878931
GenBank AAG07383.1
INSDC AAG07383.1
NCBI Locus Tag PA3996
protein_id(GenBank) gb|AAG07383.1|AE004817_7|gnl|PseudoCAP|PA3996
TIGR NTL03PA03996
UniParc UPI000012E6D0
UniProtKB Acc Q9HX25
UniProtKB ID LIPA_PSEAE
UniRef100 UniRef100_B7V9C1
UniRef50 UniRef50_Q31F42
UniRef90 UniRef90_A6V0B4

Product

Feature Type CDS
Coding Frame 1
Product Name
lipoate synthase
Product Name Confidence: Class 2
Synonyms lipoic acid synthetase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.55
Kyte-Doolittle Hydrophobicity Value -0.398
Molecular Weight (kDa) 36.7
Isoelectric Point (pI) 6.90

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 495 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001687 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3996
Search term: lis
Search term: lipoate synthase

Human Homologs

Ensembl 110, assembly GRCh38.p14
lipoic acid synthetase [Source:HGNC Symbol;Acc:HGNC:16429]
E-value: 1.2e-73
Percent Identity: 48.2

References

Escherichia coli LipA is a lipoyl synthase: in vitro biosynthesis of lipoylated pyruvate dehydrogenase complex from octanoyl-acyl carrier protein.
Miller JR, Busby RW, Jordan SW, Cheek J, Henshaw TF, Ashley GW, Broderick JB, Cronan JE Jr, Marletta MA
Biochemistry 2000 Dec 12;39(49):15166-78
PubMed ID: 11106496
Lipoic acid metabolism in Escherichia coli: isolation of null mutants defective in lipoic acid biosynthesis, molecular cloning and characterization of the E. coli lip locus, and identification of the lipoylated protein of the glycine cleavage system.
Vanden Boom TJ, Reed KE, Cronan JE Jr
J Bacteriol 1991 Oct;173(20):6411-20
PubMed ID: 1655709
The biosynthesis of lipoic acid. Cloning of lip, a lipoate biosynthetic locus of Escherichia coli.
Hayden MA, Huang I, Bussiere DE, Ashley GW
J Biol Chem 1992 May 15;267(14):9512-5
PubMed ID: 1577793
Lipoic acid metabolism in Escherichia coli: sequencing and functional characterization of the lipA and lipB genes.
Reed KE, Cronan JE Jr
J Bacteriol 1993 Mar;175(5):1325-36
PubMed ID: 8444795
Lipoic acid metabolism in Escherichia coli: the lplA and lipB genes define redundant pathways for ligation of lipoyl groups to apoprotein.
Morris TW, Reed KE, Cronan JE Jr
J Bacteriol 1995 Jan;177(1):1-10
PubMed ID: 8002607