Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5009
|
Name |
waaP
Synonym: rfaP |
Replicon | chromosome |
Genomic location | 5627865 - 5628671 (- strand) |
Transposon Mutants | 1 transposon mutants in PAO1 |
RefSeq | NP_253696.1 |
GI | 15600202 |
Affymetrix | PA5009_waaP_at |
DNASU | PaCD00008162 |
Entrez | 881540 |
GenBank | AAG08394.1 |
INSDC | AAG08394.1 |
NCBI Locus Tag | PA5009 |
protein_id(GenBank) | gb|AAG08394.1|AE004913_9|gnl|PseudoCAP|PA5009 |
TIGR | NTL03PA05010 |
UniParc | UPI00000C5EC5 |
UniProtKB Acc | Q9HUF7 |
UniProtKB ID | RFAP_PSEAE |
UniRef100 | UniRef100_Q9HUF7 |
UniRef50 | UniRef50_Q9HUF7 |
UniRef90 | UniRef90_Q9HUF7 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
lipopolysaccharide kinase WaaP
|
Synonyms |
RfaP protein heptose kinase WaaP |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 13.87 |
Kyte-Doolittle Hydrophobicity Value | -0.553 |
Molecular Weight (kDa) | 31.3 |
Isoelectric Point (pI) | 10.37 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6DFL | HYDROLASE | 05/15/18 | WaaP in complex with acyl carrier protein | Escherichia coli; Pseudomonas aeruginosa | 2.396 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 25 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004616 (537 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5009
Search term: waaP
Search term: lipopolysaccharide kinase WaaP
|
WaaP of Pseudomonas aeruginosa is a novel eukaryotic type protein-tyrosine kinase as well as a sugar kinase essential for the biosynthesis of core lipopolysaccharide.
Zhao X, Lam JS
J Biol Chem 2002 Feb 15;277(7):4722-30
PubMed ID: 11741974
|
[How signal transduction systems look like after recent dramatic progress].
Chida K, Fukui Y, Kato S, Itoh H
Tanpakushitsu Kakusan Koso 1999 Dec;44(16):2535-64
PubMed ID: 10589288
|
Purification and characterization of WaaP from Escherichia coli, a lipopolysaccharide kinase essential for outer membrane stability.
Yethon JA, Whitfield C
J Biol Chem 2001 Feb 23;276(8):5498-504
PubMed ID: 11069912
|
Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12.
Parker CT, Pradel E, Schnaitman CA
J Bacteriol 1992 Feb;174(3):930-4
PubMed ID: 1732225
|
Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica.
Heinrichs DE, Yethon JA, Whitfield C
Mol Microbiol 1998 Oct;30(2):221-32
PubMed ID: 9791168
|