Pseudomonas aeruginosa PAO1, PA5274 (rnk)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5274
Name
rnk
Replicon chromosome
Genomic location 5940020 - 5940424 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253961.1
GI 15600467
Affymetrix PA5274_rnk_at
DNASU PaCD00008277
Entrez 877820
GenBank AAG08659.1
INSDC AAG08659.1
NCBI Locus Tag PA5274
protein_id(GenBank) gb|AAG08659.1|AE004940_3|gnl|PseudoCAP|PA5274
TIGR NTL03PA05275
UniParc UPI00000C5F83
UniProtKB Acc Q9HTS6
UniProtKB ID Q9HTS6_PSEAE
UniRef100 UniRef100_W0Z362
UniRef50 UniRef50_A6VE47
UniRef90 UniRef90_A6VE47

Product

Feature Type CDS
Coding Frame 1
Product Name
nucleoside diphosphate kinase regulator
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.80
Kyte-Doolittle Hydrophobicity Value -0.103
Molecular Weight (kDa) 14.4
Isoelectric Point (pI) 4.18

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 199 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004852 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5274
Search term: rnk

Human Homologs

References

The Escherichia coli genes sspA and rnk can functionally replace the Pseudomonas aeruginosa alginate regulatory gene algR2.
Schlictman D, Shankar S, Chakrabarty AM
Mol Microbiol 1995 Apr;16(2):309-20
PubMed ID: 7565093
Regulation of nucleoside diphosphate kinase and an alternative kinase in Escherichia coli: role of the sspA and rnk genes in nucleoside triphosphate formation.
Shankar S, Schlictman D, Chakrabarty AM
Mol Microbiol 1995 Sep;17(5):935-43
PubMed ID: 8596442