Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0300
|
Name |
spuD
Synonym: potF2 |
Replicon | chromosome |
Genomic location | 338437 - 339540 (+ strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_248991.1 |
GI | 15595497 |
Affymetrix | PA0300_potF2_at |
Entrez | 880655 |
GenBank | AAG03689.1 |
INSDC | AAG03689.1 |
NCBI Locus Tag | PA0300 |
protein_id(GenBank) | gb|AAG03689.1|AE004468_1|gnl|PseudoCAP|PA0300 |
TIGR | NTL03PA00301 |
UniParc | UPI00000C4FEF |
UniProtKB Acc | Q9I6J1 |
UniProtKB ID | SPUD_PSEAE |
UniRef100 | UniRef100_Q02UB7 |
UniRef50 | UniRef50_P31133 |
UniRef90 | UniRef90_Q02UB7 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
polyamine transport protein
|
Synonyms | |
Evidence for Translation |
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 1.14 |
Kyte-Doolittle Hydrophobicity Value | -0.292 |
Molecular Weight (kDa) | 40.6 |
Isoelectric Point (pI) | 7.59 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
3TTK | TRANSPORT PROTEIN | 09/14/11 | Crystal structure of apo-SpuD | Pseudomonas aeruginosa | 2.97 | X-RAY DIFFRACTION | 99.7 |
3TTM | TRANSPORT PROTEIN | 09/15/11 | Crystal structure of SpuD in complex with putrescine | Pseudomonas aeruginosa | 2 | X-RAY DIFFRACTION | 99.7 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 306 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000297 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0300
Search term: spuD
Search term: polyamine transport protein
|
Proteome analysis reveals adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung environment.
Sriramulu DD, Nimtz M, Romling U
Proteomics 2005 Sep;5(14):3712-21
PubMed ID: 16097035
|
Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome.
Pistocchi R, Kashiwagi K, Miyamoto S, Nukui E, Sadakata Y, Kobayashi H, Igarashi K
J Biol Chem 1993 Jan 5;268(1):146-52
PubMed ID: 8416922
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|