Pseudomonas aeruginosa PAO1, PA0170 (siaC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0170
Name
siaC
Replicon chromosome
Genomic location 193799 - 194179 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
CommentThe phosphorylation status of T68 of the SiaC protein is balanced by the phosphatase activity of SiaA (PA0172) and kinase activity of SiaB (PA0171)[PMID:33156827]
CommentThe SiaC protein shares structural similarities with anti-sigma factor antagonists (PDB-ID: 6K4F)[PMID:33156827]
CommentThe SiaABC threonine phosphorylation pathway controls aggregate/biofilm formation in response to carbon availability (glucose, succinate, ethanol, 2,3-butanediol)[PMID:33156827]

Cross-References

RefSeq NP_248860.1
GI 15595368
Affymetrix PA0170_at
DNASU PaCD00006075
Entrez 878493
GenBank AAG03560.1
INSDC AAG03560.1
NCBI Locus Tag PA0170
protein_id(GenBank) gb|AAG03560.1|AE004455_1|gnl|PseudoCAP|PA0170
TIGR NTL03PA00171
UniParc UPI00000C4F79
UniProtKB Acc Q9I6W2
UniProtKB ID Q9I6W2_PSEAE
UniRef100 UniRef100_Q02UQ8
UniRef50 UniRef50_Q02UQ8
UniRef90 UniRef90_Q02UQ8

Product

Feature Type CDS
Coding Frame 1
Product Name
SiaC
Product Name Confidence: Class 1
Synonyms putative anti-sigma factor antagonist
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.07
Kyte-Doolittle Hydrophobicity Value -0.552
Molecular Weight (kDa) 14.6
Isoelectric Point (pI) 4.29

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6KKO GENE REGULATION 07/26/19 The crystal structure of SiaB-SiaC complex from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.099 X-RAY DIFFRACTION 100.0
6KKP GENE REGULATION 07/26/19 The crystal structure of apo-SiaC from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.5 X-RAY DIFFRACTION 100.0
6K4F BIOSYNTHETIC PROTEIN 05/23/19 SiaC of Pseudomonas aeruginosa Pseudomonas aeruginosa 1.735 X-RAY DIFFRACTION 100.0
7E6G DE NOVO PROTEIN 02/22/21 Crystal structure of diguanylate cyclase SiaD in complex with its activator SiaC from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.65 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 53 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000168 (328 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0170
Search term: siaC
Search term: SiaC

Human Homologs

References

The SiaABC threonine phosphorylation pathway controls biofilm formation in response to carbon availability in Pseudomonas aeruginosa.
Poh WH, Lin J, Colley B, Müller N, Goh BC, Schleheck D, El Sahili A, Marquardt A, Liang Y, Kjelleberg S, Lescar J, Rice SA, Klebensberger J
PLoS One 2020;15(11):e0241019
PubMed ID: 33156827