Pseudomonas aeruginosa PAO1, PA0425 (mexA)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA0425
|
| Name |
mexA
|
| Replicon | chromosome |
| Genomic location | 472024 - 473175 (+ strand) |
| Transposon Mutants | 2 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_249116.1 |
| GI | 15595622 |
| Affymetrix | PA0425_mexA_at |
| Entrez | 877855 |
| GenBank | AAG03814.1 |
| INSDC | AAD45627.1 |
| NCBI Locus Tag | PA0425 |
| protein_id(GenBank) | gb|AAG03814.1|AE004479_9|gnl|PseudoCAP|PA0425 |
| TIGR | NTL03PA00426 |
| UniParc | UPI000012F021 |
| UniProtKB Acc | P52477 |
| UniProtKB ID | MEXA_PSEAE |
| UniRef100 | UniRef100_P52477 |
| UniRef50 | UniRef50_P52477 |
| UniRef90 | UniRef90_P52477 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
Resistance-Nodulation-Cell Division (RND) multidrug efflux membrane fusion protein MexA precursor
|
| Synonyms |
RND multidrug efflux membrane fusion protein MexA precursor |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (40.97 kDa, PMID:21751344)
Detected using 2-DE, MALDI-TOF/MS analysis (40.97 kDa, pI 8.8, PMID:16335974)
|
| Charge (pH 7) | 3.22 |
| Kyte-Doolittle Hydrophobicity Value | -0.229 |
| Molecular Weight (kDa) | 41.0 |
| Isoelectric Point (pI) | 9.15 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
Detected in sarcosine-insoluble outer membrane fraction using 2-DE, MALDI-TOF/MS analysis (PMID:16335974)
|
AMR gene predictions from CARD database
Identified using the Resistance Gene Identifier (RGI)
| Model Name | Definition | Accession | Model Type (?) | SNP | Cutoff | Bit Score | Percent Identity | CARD Version |
| MexA | MexA is the membrane fusion protein of the MexAB-OprM multidrug efflux complex. [PMID:15117957] | ARO:3000377 | protein homolog model | Perfect | 716.1 | 100.0 | 3.2.5 |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 6TA6 | ANTIMICROBIAL PROTEIN | 10/29/19 | MexAB assembly of the Pseudomonas MexAB-OprM efflux pump reconstituted in nanodiscs | Pseudomonas aeruginosa | 3.2 | ELECTRON MICROSCOPY | 100.0 |
| 1T5E | TRANSPORT PROTEIN | 05/04/04 | The structure of MexA | Pseudomonas aeruginosa | 3 | X-RAY DIFFRACTION | 100.0 |
| 6TA5 | ANTIMICROBIAL PROTEIN | 10/29/19 | OprM-MexA complex from the MexAB-OprM Pseudomonas aeruginosa whole assembly reconstituted in nanodiscs | Pseudomonas aeruginosa | 3.2 | ELECTRON MICROSCOPY | 100.0 |
Drugs Targeting this Protein
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
| Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
| Glycerin | DB09462 | DrugBank | 5.1.4 | P52477 | Multidrug resistance protein MexA | 100.0 | 383 | 2.9e-204 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 394 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000416 (526 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA0425
Search term: mexA
|
Human Homologs
References
|
Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdine.
Poole K, Heinrichs DE, Neshat S
Mol Microbiol 1993 Nov;10(3):529-44
PubMed ID: 7968531
|
|
Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa.
Li XZ, Nikaido H, Poole K
Antimicrob Agents Chemother 1995 Sep;39(9):1948-53
PubMed ID: 8540696
|
|
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
|
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|