Pseudomonas aeruginosa PAO1, PA0441 (dht)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0441
Name
dht
Synonym: hyuA
Replicon chromosome
Genomic location 494816 - 496255 (- strand)
Transposon Mutants 10 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq NP_249132.1
GI 15595638
Affymetrix PA0441_at
Entrez 882285
GenBank AAG03830.1
INSDC AAG03830.1
NCBI Locus Tag PA0441
protein_id(GenBank) gb|AAG03830.1|AE004481_6|gnl|PseudoCAP|PA0441
TIGR NTL03PA00442
UniParc UPI00000C5058
UniProtKB Acc Q9I676
UniProtKB ID HYDA_PSEAE
UniRef100 UniRef100_Q9I676
UniRef50 UniRef50_Q59699
UniRef90 UniRef90_Q9I676

Product

Feature Type CDS
Coding Frame 1
Product Name
dihydropyrimidinase
Product Name Confidence: Class 2
Synonyms (DHPase)
D-hydantoinase, 5,6-dihydropyrimidine amidohydrolase
Evidence for Translation
Charge (pH 7) -9.36
Kyte-Doolittle Hydrophobicity Value -0.171
Molecular Weight (kDa) 52.2
Isoelectric Point (pI) 6.17

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6KLK HYDROLASE 07/30/19 Crystal structure of the Pseudomonas aeruginosa dihydropyrimidinase complexed with 5-FU Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.759 X-RAY DIFFRACTION 100.0
6AJD HYDROLASE 08/27/18 Crystal structure of a monometallic dihydropyrimidinase from Pseudomonas aeruginosa PAO1 reveals no lysine carbamylation within the active site Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.223 X-RAY DIFFRACTION 100.0
5YKD HYDROLASE 10/14/17 Crystal structure of dihydropyrimidinase from Pseudomonas aeruginosa PAO1 at 2.17 angstrom resolution Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.17 X-RAY DIFFRACTION 100.0
5E5C HYDROLASE 10/08/15 Crystal structure of dihydropyrimidinase from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 2.1 X-RAY DIFFRACTION 100.0
7E3U HYDROLASE 02/09/21 Crystal structure of the Pseudomonas aeruginosa dihydropyrimidinase complexed with 5-AU Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.159 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 561 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000432 (819 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0441
Search term: dht

Human Homologs

Ensembl 110, assembly GRCh38.p14
dihydropyrimidinase [Source:HGNC Symbol;Acc:HGNC:3013]
E-value: 9.8e-138
Percent Identity: 51.2

References

Pyrimidine catabolism in Pseudomonas aeruginosa.
Kim S, West TP
FEMS Microbiol Lett 1991 Jan 15;61(2-3):175-9
PubMed ID: 1903745
Cloning, sequencing, and expression in Escherichia coli of the D-hydantoinase gene from Pseudomonas putida and distribution of homologous genes in other microorganisms.
LaPointe G, Viau S, LeBlanc D, Robert N, Morin A
Appl Environ Microbiol 1994 Mar;60(3):888-95
PubMed ID: 8161181
Identification of the open reading frame for the Pseudomonas putida D-hydantoinase gene and expression of the gene in Escherichia coli.
Chien HR, Jih YL, Yang WY, Hsu WH
Biochim Biophys Acta 1998 Jan 7;1395(1):68-77
PubMed ID: 9434154