Pseudomonas aeruginosa PAO1, PA0441 (dht)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest |
| Locus Tag |
PA0441
|
| Name |
dht
Synonym: hyuA |
| Replicon | chromosome |
| Genomic location | 494816 - 496255 (- strand) |
| Transposon Mutants | 10 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 4 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_249132.1 |
| GI | 15595638 |
| Affymetrix | PA0441_at |
| Entrez | 882285 |
| GenBank | AAG03830.1 |
| INSDC | AAG03830.1 |
| NCBI Locus Tag | PA0441 |
| protein_id(GenBank) | gb|AAG03830.1|AE004481_6|gnl|PseudoCAP|PA0441 |
| TIGR | NTL03PA00442 |
| UniParc | UPI00000C5058 |
| UniProtKB Acc | Q9I676 |
| UniProtKB ID | HYDA_PSEAE |
| UniRef100 | UniRef100_Q9I676 |
| UniRef50 | UniRef50_Q59699 |
| UniRef90 | UniRef90_Q9I676 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
dihydropyrimidinase
|
| Synonyms |
(DHPase) D-hydantoinase, 5,6-dihydropyrimidine amidohydrolase |
| Evidence for Translation | |
| Charge (pH 7) | -9.36 |
| Kyte-Doolittle Hydrophobicity Value | -0.171 |
| Molecular Weight (kDa) | 52.2 |
| Isoelectric Point (pI) | 6.17 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold Protein Structure Database
Available for P. aeruginosa PAO1.
| View predicted 3D structure in AlphaFold DB |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 6KLK | HYDROLASE | 07/30/19 | Crystal structure of the Pseudomonas aeruginosa dihydropyrimidinase complexed with 5-FU | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.759 | X-RAY DIFFRACTION | 100.0 |
| 5YKD | HYDROLASE | 10/14/17 | Crystal structure of dihydropyrimidinase from Pseudomonas aeruginosa PAO1 at 2.17 angstrom resolution | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2.17 | X-RAY DIFFRACTION | 100.0 |
| 7E3U | HYDROLASE | 02/09/21 | Crystal structure of the Pseudomonas aeruginosa dihydropyrimidinase complexed with 5-AU | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2.159 | X-RAY DIFFRACTION | 100.0 |
| 5E5C | HYDROLASE | 10/08/15 | Crystal structure of dihydropyrimidinase from Pseudomonas aeruginosa PAO1 | Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) | 2.1 | X-RAY DIFFRACTION | 100.0 |
| 6AJD | HYDROLASE | 08/27/18 | Crystal structure of a monometallic dihydropyrimidinase from Pseudomonas aeruginosa PAO1 reveals no lysine carbamylation within the active site | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2.223 | X-RAY DIFFRACTION | 100.0 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 561 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000432 (845 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA0441
Search term: dht
Search term: dihydropyrimidinase
|
Human Homologs
|
Ensembl
107, assembly
GRCh38.p13
|
dihydropyrimidinase [Source:HGNC Symbol;Acc:HGNC:3013]
E-value:
9.5e-138
Percent Identity:
51.2
|
References
|
Pyrimidine catabolism in Pseudomonas aeruginosa.
Kim S, West TP
FEMS Microbiol Lett 1991 Jan 15;61(2-3):175-9
PubMed ID: 1903745
|
|
Cloning, sequencing, and expression in Escherichia coli of the D-hydantoinase gene from Pseudomonas putida and distribution of homologous genes in other microorganisms.
LaPointe G, Viau S, LeBlanc D, Robert N, Morin A
Appl. Environ. Microbiol. 1994 Mar;60(3):888-95
PubMed ID: 8161181
|
|
Identification of the open reading frame for the Pseudomonas putida D-hydantoinase gene and expression of the gene in Escherichia coli.
Chien HR, Jih YL, Yang WY, Hsu WH
Biochim. Biophys. Acta 1998 Jan;1395(1):68-77
PubMed ID: 9434154
|