Pseudomonas aeruginosa PAO1, PA0504 (bioD)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest |
| Locus Tag |
PA0504
|
| Name |
bioD
|
| Replicon | chromosome |
| Genomic location | 563549 - 564235 (+ strand) |
| Transposon Mutants | 2 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_249195.1 |
| GI | 15595701 |
| Affymetrix | PA0504_bioD_at |
| DNASU | PaCD00006212 |
| Entrez | 878220 |
| GenBank | AAG03893.1 |
| INSDC | AAG03893.1 |
| NCBI Locus Tag | PA0504 |
| protein_id(GenBank) | gb|AAG03893.1|AE004487_7|gnl|PseudoCAP|PA0504 |
| TIGR | NTL03PA00505 |
| UniParc | UPI000012697C |
| UniProtKB Acc | Q9I614 |
| UniProtKB ID | BIOD_PSEAE |
| UniRef100 | UniRef100_Q9I614 |
| UniRef50 | UniRef50_Q9I614 |
| UniRef90 | UniRef90_Q9I614 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
dethiobiotin synthase
|
| Synonyms |
DTB synthetase DTBS dethiobiotin synthetase |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | -4.92 |
| Kyte-Doolittle Hydrophobicity Value | 0.501 |
| Molecular Weight (kDa) | 23.3 |
| Isoelectric Point (pI) | 5.26 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold Protein Structure Database
Available for P. aeruginosa PAO1.
| View predicted 3D structure in AlphaFold DB |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 405 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000491 (555 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA0504
Search term: bioD
Search term: dethiobiotin synthase
|
Human Homologs
References
|
The purification and properties of dethiobiotin synthetase.
Krell K, Eisenberg MA
J Biol Chem 1970 Dec 25;245(24):6558-66
PubMed ID: 4921568
|
|
Sequence and crystallization of Escherichia coli dethiobiotin synthetase, the penultimate enzyme of biotin biosynthesis.
Alexeev D, Bury SM, Boys CW, Turner MA, Sawyer L, Ramsey AJ, Baxter HC, Baxter RL
J. Mol. Biol. 1994 Jan;235(2):774-6
PubMed ID: 8289297
|
|
Crystal structure of an ATP-dependent carboxylase, dethiobiotin synthetase, at 1.65 A resolution.
Huang W, Lindqvist Y, Schneider G, Gibson KJ, Flint D, Lorimer G
Structure 1994 May;2(5):407-14
PubMed ID: 8081756
|
|
Active site mutants of Escherichia coli dethiobiotin synthetase: effects of mutations on enzyme catalytic and structural properties.
Yang G, Sandalova T, Lohman K, Lindqvist Y, Rendina AR
Biochemistry 1997 Apr;36(16):4751-60
PubMed ID: 9125495
|