Pseudomonas aeruginosa PAO1, PA0510 (nirE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0510
Name
nirE
Synonym: nirE
Replicon chromosome
Genomic location 572583 - 573422 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249201.1
GI 15595707
Affymetrix PA0510_at
Entrez 881871
GenBank AAG03899.1
INSDC AAG03899.1
NCBI Locus Tag PA0510
protein_id(GenBank) gb|AAG03899.1|AE004488_3|gnl|PseudoCAP|PA0510
TIGR NTL03PA00511
UniParc UPI00000D4275
UniProtKB Acc G3XD80
UniProtKB ID G3XD80_PSEAE
UniRef100 UniRef100_W0YLY2
UniRef50 UniRef50_W6LVC5
UniRef90 UniRef90_V6A922

Product

Feature Type CDS
Coding Frame 1
Product Name
NirE
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.26
Kyte-Doolittle Hydrophobicity Value 0.159
Molecular Weight (kDa) 29.7
Isoelectric Point (pI) 7.09

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2YBO TRANSFERASE 03/08/11 The x-ray structure of the SAM-dependent uroporphyrinogen III methyltransferase NirE from Pseudomonas aeruginosa in complex with SAH PSEUDOMONAS AERUGINOSA 2 X-RAY DIFFRACTION 100.0
2YBQ TRANSFERASE 03/09/11 The x-ray structure of the SAM-dependent uroporphyrinogen III methyltransferase NirE from Pseudomonas aeruginosa in complex with SAH and uroporphyrinogen III PSEUDOMONAS AERUGINOSA 2 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 537 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000495 (363 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0510
Search term: nirE
Search term: NirE

Human Homologs

References

The Pseudomonas aeruginosa nirE gene encodes the S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase required for heme d(1) biosynthesis.
Storbeck S, Walther J, Müller J, Parmar V, Schiebel HM, Kemken D, Dülcks T, Warren MJ, Layer G
FEBS J. 2009 Oct;276(20):5973-82
PubMed ID: 19754882
Gene cluster for dissimilatory nitrite reductase (nir) from Pseudomonas aeruginosa: sequencing and identification of a locus for heme d1 biosynthesis.
Kawasaki S, Arai H, Kodama T, Igarashi Y
J. Bacteriol. 1997 Jan;179(1):235-42
PubMed ID: 8982003