Pseudomonas aeruginosa PAO1, PA2253 (ansA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2253
Name
ansA
Replicon chromosome
Genomic location 2480844 - 2481830 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250943.1
GI 15597449
Affymetrix PA2253_ansA_at
Entrez 878133
GenBank AAG05641.1
INSDC AAG05641.1
NCBI Locus Tag PA2253
protein_id(GenBank) gb|AAG05641.1|AE004651_3|gnl|PseudoCAP|PA2253
TIGR NTL03PA02253
UniParc UPI00000C5630
UniProtKB Acc Q9I1L6
UniProtKB ID Q9I1L6_PSEAE
UniRef100 UniRef100_W0YPK0
UniRef50 UniRef50_E2Y049
UniRef90 UniRef90_B7V970

Product

Feature Type CDS
Coding Frame 1
Product Name
L-asparaginase I
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.93
Kyte-Doolittle Hydrophobicity Value 0.116
Molecular Weight (kDa) 34.8
Isoelectric Point (pI) 5.34

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 358 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003236 (524 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2253
Search term: ansA
Search term: L-asparaginase I

Human Homologs

Ensembl 110, assembly GRCh38.p14
asparaginase [Source:HGNC Symbol;Acc:HGNC:20123]
E-value: 6.2e-35
Percent Identity: 32.6
Ensembl 110, assembly GRCh38.p14
asparaginase [Source:HGNC Symbol;Acc:HGNC:20123]
E-value: 6.2e-35
Percent Identity: 32.6

References

Structure and expression in Escherichia coli K-12 of the L-asparaginase I-encoding ansA gene and its flanking regions.
Jerlström PG, Bezjak DA, Jennings MP, Beacham IR
Gene 1989 May 15;78(1):37-46
PubMed ID: 2670682