Pseudomonas aeruginosa PAO1, PA2442 (gcvT2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2442
Name
gcvT2
Replicon chromosome
Genomic location 2739762 - 2740883 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251132.1
GI 15597638
Affymetrix PA2442_gcvT2_at
DNASU PaCD00007062
Entrez 882934
GenBank AAG05830.1
INSDC AAG05830.1
NCBI Locus Tag PA2442
protein_id(GenBank) gb|AAG05830.1|AE004671_6|gnl|PseudoCAP|PA2442
TIGR NTL03PA02442
UniParc UPI00000C56D6
UniProtKB Acc Q9I140
UniProtKB ID Q9I140_PSEAE
UniRef100 UniRef100_Q9I140
UniRef50 UniRef50_Q0BYP4
UniRef90 UniRef90_Q9I140

Product

Feature Type CDS
Coding Frame 1
Product Name
glycine cleavage system protein T2
Product Name Confidence: Class 2
Synonyms aminomethyltransferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -3.74
Kyte-Doolittle Hydrophobicity Value 0.082
Molecular Weight (kDa) 39.9
Isoelectric Point (pI) 6.35

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 485 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003096 (502 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2442
Search term: gcvT2

Human Homologs

Ensembl 110, assembly GRCh38.p14
aminomethyltransferase [Source:HGNC Symbol;Acc:HGNC:473]
E-value: 4.0e-78
Percent Identity: 48.5

References

Structure and chromosomal localization of the aminomethyltransferase gene (AMT).
Nanao K, Takada G, Takahashi E, Seki N, Komatsu Y, Okamura-Ikeda K, Motokawa Y, Hayasaka K
Genomics 1994 Jan 1;19(1):27-30
PubMed ID: 8188235
T-protein of the glycine decarboxylase multienzyme complex: evidence for partial similarity to formyltetrahydrofolate synthetase.
Kopriva S, Turner SR, Rawsthorne S, Bauwe H
Plant Mol Biol 1995 Mar;27(6):1215-20
PubMed ID: 7766903
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994