Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA2741
|
Name |
rplT
|
Replicon | chromosome |
Genomic location | 3103643 - 3103999 (- strand) |
RefSeq | NP_251431.1 |
GI | 15597937 |
Affymetrix | PA2741_rplT_at |
DNASU | PaCD00007186 |
Entrez | 882982 |
GenBank | AAG06129.1 |
INSDC | AAG06129.1 |
NCBI Locus Tag | PA2741 |
protein_id(GenBank) | gb|AAG06129.1|AE004702_6|gnl|PseudoCAP|PA2741 |
TIGR | NTL03PA02741 |
UniParc | UPI0000133D1E |
UniProtKB Acc | Q9I0A2 |
UniProtKB ID | RL20_PSEAE |
UniRef100 | UniRef100_A6V488 |
UniRef50 | UniRef50_A0QHC1 |
UniRef90 | UniRef90_C1DF45 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
50S ribosomal protein L20
|
Synonyms | |
Evidence for Translation |
Detected using LC-MS/MS (13.36 kDa, PMID:21751344)
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 26.22 |
Kyte-Doolittle Hydrophobicity Value | -0.355 |
Molecular Weight (kDa) | 13.4 |
Isoelectric Point (pI) | 12.25 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6SPD | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 50s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.28 | ELECTRON MICROSCOPY | 100.0 |
7UNR | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
7UNW | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.6 | ELECTRON MICROSCOPY | 100.0 |
7UNU | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
7UNV | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.7 | ELECTRON MICROSCOPY | 100.0 |
6SPG | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.34 | ELECTRON MICROSCOPY | 100.0 |
6SPF | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate | Pseudomonas aeruginosa | 2.89 | ELECTRON MICROSCOPY | 100.0 |
6SPB | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 | Pseudomonas aeruginosa | 2.82 | ELECTRON MICROSCOPY | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 591 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002830 (529 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA2741
Search term: rplT
Search term: 50S ribosomal protein L20
|
Ensembl
110, assembly
GRCh38.p14
|
mitochondrial ribosomal protein L20 [Source:HGNC Symbol;Acc:HGNC:14478]
E-value:
3.8e-11
Percent Identity:
38.6
|
Attenuation control of the Escherichia coli phenylalanyl-tRNA synthetase operon.
Springer M, Mayaux JF, Fayat G, Plumbridge JA, Graffe M, Blanquet S, Grunberg-Manago M
J Mol Biol 1985 Feb 20;181(4):467-78
PubMed ID: 3158742
|
Suppression of a sensor kinase-dependent phenotype in Pseudomonas syringae by ribosomal proteins L35 and L20.
Kitten T, Willis DK
J Bacteriol 1996 Mar;178(6):1548-55
PubMed ID: 8626280
|
Physiological effects of translation initiation factor IF3 and ribosomal protein L20 limitation in Escherichia coli.
Olsson CL, Graffe M, Springer M, Hershey JW
Mol Gen Genet 1996 Apr 10;250(6):705-14
PubMed ID: 8628231
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|